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  • %0 ART
  • %T The persistence, dissemination, and visceralization tendency of Leishmania major in Syrian hamsters
  • %A SOLIMAN Maha F. M.
  • %G 0001-706X
  • %I Elsevier
  • %C Oxford, ROYAUME-UNI
  • %D 2006
  • %V 97
  • %N 2
  • %P 146-150
  • %P 5
  • %O Anglais
  • %K Molecular biology
  • %K Biologie moléculaire
  • %K Vertebrata
  • %K Vertebrata
  • %K Mammalia
  • %K Mammalia
  • %K Rodentia
  • %K Rodentia
  • %K Asia
  • %K Asie
  • %K Protozoa
  • %K Protozoa
  • %K Kinetoplastida
  • %K Kinetoplastida
  • %K Viscus
  • %K Viscère
  • %K Experimental disease
  • %K Pathologie expérimentale
  • %K Tropical medicine
  • %K Médecine tropicale
  • %K Tissue
  • %K Tissu
  • %K Hamster
  • %K Hamster
  • %K Animal
  • %K Animal
  • %K Syria
  • %K Syrie
  • %K Leishmania major
  • %K Leishmania major
  • %K Dissemination
  • %K Dissémination
  • %K Polymerase chain reaction
  • %K Réaction chaîne polymérase
  • %K Smear
  • %K Frottis
  • %K Leishmania major
  • %K Hamsters
  • %K Tissue smears
  • %K Polymerase chain reaction (PCR)
  • %K Visceralization
  • %K Dissemination
  • %X I monitored the persistence, dissemination, and the possible visceralization tendency ofLeishmania major, the causative parasite of cutaneous leishmaniasis in North Africa and the Middle East in Syrian hamsters (Mesocricetus auratus). Hamsters were inoculated subcutaneously in the hind footpad, with 1 x 10[6] L. major metacyclic promastigotes and were sacrificed at months 1, 3, 6 and 10 post-infection (pi). Skin lesions, blood, spleens, livers and kidneys were screened by both Giemsa-stained smears and a polymerase chain reaction (PCR) for detection ofL. major amastigotes. A few weeks pi, 61.7% of the inoculated hamsters developed a cutaneous lesion only at the inoculation site, while 38.3% of them developed non-self-healed lesions at sites distant from the inoculation site. PCR identified all the positive stained smears as well as false-negative ones, indicating that PCR was more sensitive than stained smears. The results confirmed the dissemination and persistence ofL. major amastigotes in all tissues examined, except the kidneys, for a period extending to 10 months, only in those hamsters suffering from disseminated cutaneous lesions. Parasite DNA was detected in the bloods starting from the first month pi and starting from month 3 pi in the spleens and livers. Some, but not all, the animals with disseminated infections proved to be positive for parasite DNA in their organs. Persistence of the L. major amastigotes in the tissues differed from those of other species causing visceral diseases. These findings demonstrate a possible visceralization tendency for L. major previously recorded for L. tropica and L. mexicana. 
  • %S Acta tropica

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