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Titre du document / Document title

Elevated plasma endothelial microparticles in multiple sclerosis

Auteur(s) / Author(s)

MINAGAR A. (1) ; JY W. (2) ; JIMENEZ J. J. (2) ; SHEREMATA W. A. (1) ; MAURO L. M. (2) ; MAO W. W. (2) ; HORSTMAN L. L. (2) ; AHN Y. S. (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Neurology, University of Miami, ETATS-UNIS
(2) Department of Medicine, Wallace H. Coulter Platelet Laboratory, University of Miami, FL, ETATS-UNIS

Résumé / Abstract

-Objective: To assess endothelial dysfunction in patients with MS and to investigate whether plasma from patients with MS induces endothelial cell dysfunction in vitro. Background: Endothelial cell dysfunction may contribute to the pathogenesis of MS. Elevations of soluble adhesion molecules intracellular adhesion molecule, vascular cell adhesion molecule, and platelet-endothelial cell adhesion molecule-1 (CD31) have been reported as markers of blood-brain barrier (BBB) damage in MS, but direct assay of endothelium has been difficult. Endothelial cells release microparticles <∼1.5 μm (EMP) during activation or apoptosis. The authors developed a flow cytometric assay of EMP and studied EMP as markers of endothelial damage in MS. Methods: Platelet-poor plasma (PPP) from 50 patients with MS (30 in exacerbation and 20 in remission) and 48 controls were labeled with fluorescein isothiocyanate (FITC)-conjugated anti-CD31 and anti-CD51 (vitronectin receptor) antibodies, and two classes of EMP (CD31+ and CD51+) were assayed by flow cytometry. For in vitro studies, patients' plasma was added to the microvascular endothelial cell (MVEC) culture and release of CD31+ and CD51+ EMP were measured in the supernatant. Results: Plasma from patients in exacerbation had 2.85-fold elevation of CD31+ EMP as compared with healthy controls, returning to near control value during remission. The CD31+ EMP concentration showed a positive association with gadolinium enhancement in patients with MS. In contrast, CD51+ EMP remained elevated in both exacerbation and remission. This suggests that CD31+ EMP is a marker of acute injury, whereas CD51+ EMP reflects chronic injury of endothelium. MS plasma induced release of both CD31+ and CD51+ EMP from MVEC culture in vitro. Conclusion: Endothelial dysfunction is evident during exacerbation of MS, evidenced by shedding of EMP expressing PECAM-1 (CD31). The in vitro data indicate contribution of one or more plasma factors in endothelial dysfunction of MS.

Revue / Journal Title

Neurology    ISSN  0028-3878   CODEN NEURAI 

Source / Source

2001, vol. 56, no10, pp. 1319-1324 (27 ref.)

Langue / Language

Anglais

Editeur / Publisher

Lippincott Williams & Wilkins, Hagerstown, MD, ETATS-UNIS  (1951) (Revue)

Mots-clés anglais / English Keywords

Multiple sclerosis

;

Blood plasma

;

Endothelium

;

In vitro

;

Pathophysiology

;

Human

;

Nervous system diseases

;

Central nervous system disease

;

Inflammatory disease

;

Mots-clés français / French Keywords

Sclérose en plaque

;

Plasma sanguin

;

Endothélium

;

In vitro

;

Physiopathologie

;

Homme

;

Microparticule

;

Système nerveux pathologie

;

Système nerveux central pathologie

;

Maladie inflammatoire

;

Mots-clés espagnols / Spanish Keywords

Esclerosis en placa

;

Plasma sanguíneo

;

Endotelio

;

In vitro

;

Fisiopatología

;

Hombre

;

Sistema nervioso patología

;

Sistema nervosio central patología

;

Enfermedad inflamatoria

;

Localisation / Location

INIST-CNRS, Cote INIST : 6345, 35400009895054.0120

Nº notice refdoc (ud4) : 997998



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