Titre du document / Document title
Elevated plasma endothelial microparticles in multiple sclerosis
Auteur(s) / Author(s)
MINAGAR A. (1) ;
JY W. (2) ;
JIMENEZ J. J. (2) ;
SHEREMATA W. A. (1) ;
MAURO L. M. (2) ;
MAO W. W. (2) ;
HORSTMAN L. L. (2) ;
AHN Y. S. (2) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Neurology, University of Miami, ETATS-UNIS
(2) Department of Medicine, Wallace H. Coulter Platelet Laboratory, University of Miami, FL, ETATS-UNIS
Résumé / Abstract
-Objective: To assess endothelial dysfunction in patients with MS and to investigate whether plasma from patients with MS induces endothelial cell dysfunction in vitro. Background: Endothelial cell dysfunction may contribute to the pathogenesis of MS. Elevations of soluble adhesion molecules intracellular adhesion molecule, vascular cell adhesion molecule, and platelet-endothelial cell adhesion molecule-1 (CD31) have been reported as markers of blood-brain barrier (BBB) damage in MS, but direct assay of endothelium has been difficult. Endothelial cells release microparticles <∼1.5 μm (EMP) during activation or apoptosis. The authors developed a flow cytometric assay of EMP and studied EMP as markers of endothelial damage in MS. Methods: Platelet-poor plasma (PPP) from 50 patients with MS (30 in exacerbation and 20 in remission) and 48 controls were labeled with fluorescein isothiocyanate (FITC)-conjugated anti-CD31 and anti-CD51 (vitronectin receptor) antibodies, and two classes of EMP (CD31+ and CD51+) were assayed by flow cytometry. For in vitro studies, patients' plasma was added to the microvascular endothelial cell (MVEC) culture and release of CD31+ and CD51+ EMP were measured in the supernatant. Results: Plasma from patients in exacerbation had 2.85-fold elevation of CD31+ EMP as compared with healthy controls, returning to near control value during remission. The CD31+ EMP concentration showed a positive association with gadolinium enhancement in patients with MS. In contrast, CD51+ EMP remained elevated in both exacerbation and remission. This suggests that CD31+ EMP is a marker of acute injury, whereas CD51+ EMP reflects chronic injury of endothelium. MS plasma induced release of both CD31+ and CD51+ EMP from MVEC culture in vitro. Conclusion: Endothelial dysfunction is evident during exacerbation of MS, evidenced by shedding of EMP expressing PECAM-1 (CD31). The in vitro data indicate contribution of one or more plasma factors in endothelial dysfunction of MS.
Revue / Journal Title
Neurology
ISSN
0028-3878
CODEN NEURAI
Source / Source
2001, vol. 56, n
o10, pp. 1319-1324 (27 ref.)
Langue / Language
Anglais
Editeur / Publisher
Lippincott Williams & Wilkins, Hagerstown, MD, ETATS-UNIS
(1951)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 6345, 35400009895054.0120
Nº notice refdoc (ud4) : 997998
