Titre du document / Document title

Influence of agar and activated charcoal on uptake of gibberellin and plant morphogenesis in vitro

Auteur(s) / Author(s)

MOHAMED-YASSEEN Yasseen ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Genetic Engineering and Biotechnology Research Institute, Menoufiya University, Sadat City, P.O. Box 79/22857, EGYPTE

Résumé / Abstract

Agar and activated charcoal (AC) are commonly used in tissue culture. However, their deeper actions and functions are largely unknown. This experiment investigated the effect of agar and AC, singly and jointly, on gibberellin (GA) uptake by corn shoots. Corn seeds were germinated on Murashige and Skoog medium (MS). Shoots excised from 1-wk-old seedlings were cultured on liquid (0.0 g l^-1 agar) or solid (8 g l^-1 agar) MS containing 3 μM indole-3-acetic acid, 13.3 μM N⁶-benzyladenine, and 6000 CPM ml^-1 [³H]GA₄ as tracer. Both liquid and solid media had two treatments, one without AC and one supplemented with 5 g l^-1AC. Uptake of [³H]GA₄ and morphogenesis of corn shoots were recorded after 2 wk of culture. Corn explants cultured in AC-free media acquired high levels of [³H]GA₄ while explants from AC-containing media showed only traces of [³H]GA₄. Explants cultured in AC-free liquid medium contained about twice the amount of [³H]GA₄ as those from AC-free solid medium. Addition of agar reduced shoot length, while addition of AC increased both shoot and root length. It is concluded that: (1) agar reduced the uptake of GA₄; and (2) GAS was irreversibly adsorbed by AC, and thus became unavailable to corn explants.

Revue / Journal Title

In vitro cellular & developmental biology. Plant   ISSN 1054-5476   CODEN IVCPEO 

Source / Source

Congrès
Alternative Markers for Plant Transformation. Workshop
2001, vol. 37, n^o 2, pp. 101-132 (17 ref.), pp. 204-205

Langue / Language

Anglais

Editeur / Publisher

Cambridge University Press, Wallingford, ETATS-UNIS  (1991) (Revue)

Mots-clés anglais / English Keywords

Micropropagation ; Chemical composition ; Culture medium ; Absorption ; Morphogenesis ; Radiolabelling ; Tritium ; Rhizogenesis ; Adsorption ; Zea mays ; Agar ; Activated carbon ; Gibberellic acid ; Adenine(N⁶-benzyl) ; Growth ; Gramineae ; Monocotyledones ; Angiospermae ; Spermatophyta ; Cereal crop ; Gibberellin ; Plant growth substance ; Indoleacetic acid ; Purine derivatives ;

Mots-clés français / French Keywords

Multiplication végétative in vitro ; Composition chimique ; Milieu culture ; Absorption ; Morphogenèse ; Marquage radioisotopique ; Tritium ; Rhizogenèse ; Adsorption ; Zea mays ; Gélose ; Charbon actif ; Gibbérellique acide ; Adénine(N⁶-benzyl) ; Croissance ; Indole-3-acétique acide ; Gramineae ; Monocotyledones ; Angiospermae ; Spermatophyta ; Plante céréalière ; Gibbérelline ; Substance croissance végétal ; Indoleacétique acide ; Purine dérivé ;

Mots-clés espagnols / Spanish Keywords

Multiplicación vegetativa in vitro ; Composición química ; Medio cultivo ; Absorción ; Morfogénesis ; Marcación radioisotópica ; Tritio ; Rizogénesis ; Adsorción ; Zea mays ; Agar ; Carbón activado ; Giberelico ácido ; Adenina(N⁶-benzil) ; Crecimiento ; Gramineae ; Monocotyledones ; Angiospermae ; Spermatophyta ; Planta cerealista ; Giberelina ; Substancia crecimiento vegetal ; Indoloacetico ácido ; Purina derivado ;

Localisation / Location

INIST-CNRS, Cote INIST : 15474 A, 35400009891988.0120