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Titre du document / Document title

Characterization of a soybean [Glycine max (L.) Merr.] mutant with reduced levels of Kunitz trypsin inhibitor

Auteur(s) / Author(s)

KRISHNAN Hari B. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) USDA-ARS, Plant Genetics Research Unit and Department of Agronomy, 108W Curtis Hall University of Missouri, Columbia, MO 65211, ETATS-UNIS

Résumé / Abstract

Kunitz trypsin inhibitor, an abundant soybean [Glycine max (L.) Merr.] seed protein. has a molecular mass of 21 500 Da and is specific for serine proteases. A soybean mutant (P.I. 196168) was characterized to determine the molecular basis for reduced Kunitz trypsin inhibitor levels during seed development. Western blot analysis revealed that P.I. 196168, in comparison to Amsoy 71, accumulated low amounts of Kunitz trypsin inhibitor protein. Non-denaturing polyacrylamide enzyme activity gels indicated that Amsoy 71 seeds contained at least five distinct zones of trypsin inhibitor activity. However, P.I. 196168 contained only four zones of enzyme inhibition. The coding region of the most abundant trypsin inhibitor gene (KTi3) was isolated from Amsoy 71 and P.I. 196168 by PCR. DNA sequence comparisons of the Kunitz trypsin inhibitor coding regions revealed two deletions and one G to T transversion have occurred. These mutations introduced four stop codons in the reading frame, resulting in a truncated protein. Northern blot analysis revealed that P.I. 196168 accumulated drastically lower amounts of KTi3 mRNA when compared with Amsoy 71.

Revue / Journal Title

Plant science    ISSN  0168-9452   CODEN PLSCE4 

Source / Source

2001, vol. 160, no5, pp. 979-986 (32 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1985) (Revue)

Mots-clés anglais / English Keywords

Mutation

;

Seed

;

Biological accumulation

;

Kunitz inhibitor

;

Developmental stage

;

Gene

;

Nucleotide sequence

;

Deletion

;

Glycine max

;

Antinutrient factor

;

Animal feeding

;

Leguminosae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Storage protein

;

Serine endopeptidases

;

Peptidases

;

Hydrolases

;

Enzyme

;

Grain legume

;

Mots-clés français / French Keywords

Mutation

;

Semence

;

Accumulation biologique

;

Inhibiteur Kunitz

;

Stade développement

;

Gène

;

Séquence nucléotide

;

Délétion

;

Glycine max

;

Facteur antinutritionnel

;

Alimentation animale

;

Leguminosae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Protéine réserve

;

Serine endopeptidases

;

Peptidases

;

Hydrolases

;

Enzyme

;

Légumineuse grain

;

Mots-clés espagnols / Spanish Keywords

Mutación

;

Semilla

;

Acumulación biológica

;

Inhibidor Kunitz

;

Grado desarrollo

;

Gen

;

Secuencia nucleótido

;

Deleción

;

Glycine max

;

Factor antinutricional

;

Alimentación animal

;

Leguminosae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Proteína reserva

;

Serine endopeptidases

;

Peptidases

;

Hydrolases

;

Enzima

;

Leguminosa grano

;

Localisation / Location

INIST-CNRS, Cote INIST : 15982, 35400009509697.0220

Nº notice refdoc (ud4) : 958515



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