Titre du document / Document title

Characterization of the iontophoretic permselectivity properties of human and pig skin

Auteur(s) / Author(s)

MARRO Diego ^{(1 2)} ; GUY Richard H. ^{(1 2)} ; DELGADO-CHARRO M. Begona ^{(1 2)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Centre interuniversitaire de recherche et d'enseignement Pharmapeptides, Universities of Geneva and Lyon, Parc d'affaires international, 74166 Archamps, FRANCE
⁽²⁾ University of Geneva, Faculté des Sciences, Section de Pharmacie 30, quai E. Ansermet, 1211 Geneva, SUISSE

Résumé / Abstract

The objectives of this research were (a) to characterize the permselective properties of human and porcine skin and (b) to assess the validity of the latter as a model membrane in iontophoresis studies. The electroosmotic transport of [¹⁴C]mannitol was followed in vitro across human and porcine skin as a function of pH, in both anode-to-cathode' and cathode-to-anode' directions. At physiological pH, mannitol electrotransport dominated in the anode-to-cathode direction, clearly indicating the net negative charge and the corresponding cation-permselectivity of the skin. By lowering the pH to 3.5 the direction of electroosmosis progressively reverses, indicating that the skin is becoming net positively-charged, and thus anion-selective. The degree of permselectivity (DP) of the skin at each pH value was quantified by dividing mannitol electrotransport in the predominant direction (i.e. either anodal or cathodal) by that in the opposite sense. The net charge on the skin is zero when DP equals unity, corresponding to the isoelectric point (pI) of the membrane (∼4.4 for pig skin and ∼4.8 for human skin). The consistent pls and similar pH-dependent permselectivities observed for human and pig demonstrate that porcine skin is an appropriate model for iontophoresis studies. Finally, the characterization of the perm selective properties of human skin is crucial to optimize the iontophoresis of large peptides and uncharged species, which are transported primarily by electroosmosis.

Revue / Journal Title

Journal of controlled release   ISSN 0168-3659   CODEN JCREEC 

Source / Source

2001, vol. 70, n^o1-2, pp. 213-217 (7 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1984) (Revue)

Mots-clés anglais / English Keywords

Pharmaceutical technology ; Biological transport ; Electroosmosis ; Permeation ; Permeability ; Selectivity ; Iontophoresis ; Biomembrane ; Models ; Animal ; Pig ; Human ; Skin ; pH ; Mannitol ; Electric current ; Physicochemical properties ; Artiodactyla ; Ungulata ; Mammalia ; Vertebrata ;

Mots-clés français / French Keywords

Technologie pharmaceutique ; Transport biologique ; Electroosmose ; Perméation ; Perméabilité ; Sélectivité ; Ionophorèse ; Membrane biologique ; Modèle ; Animal ; Porc ; Homme ; Peau ; pH ; Mannitol ; Courant électrique ; Propriété physicochimique ; Artiodactyla ; Ungulata ; Mammalia ; Vertebrata ;

Mots-clés espagnols / Spanish Keywords

Tecnología farmacéutica ; Transporte biológico ; Electro-osmosis ; Permeación ; Permeabilidad ; Selectividad ; Inoforesis ; Membrana biológica ; Modelo ; Animal ; Cerdo ; Hombre ; Piel ; pH ; Manitol ; Corriente eléctrica ; Propiedad fisicoquímica ; Artiodactyla ; Ungulata ; Mammalia ; Vertebrata ;

Localisation / Location

INIST-CNRS, Cote INIST : 20704, 35400009484198.0190