Titre du document / Document title

UVA-induced oxidative damage in retinal pigment epithelial cells after H₂O₂ or sparfloxacin exposure

Auteur(s) / Author(s)

VERNA L. K. ⁽¹⁾ ; HOLMAN S. A. ⁽¹⁾ ; LEE V. C. ⁽¹⁾ ; HOH J. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Division of Biomedical Sciences, University of California, Riverside, Riverside, California, ETATS-UNIS

Résumé / Abstract

Retinal impairment is one of the leading causes of visual loss in an aging human population. To explore a possible cause for retinal damage in the human population, we have monitored DNA oxidation in human retinal pigment epithelial (RPE) cells after exposure to hydrogen peroxide (H₂O₂) or the quinolone antibacterial sparfloxacin. When H₂O₂- or sparfloxacin-exposed cells were further exposed to ultraviolet A (UVA) irradiation, oxidative damage to the DNA of these cells was greatly increased over baseline values. This RPE+pharmaceutical-UVA cell system was developed to mimic in vivo retinal degeneration, seen in mouse studies using quinolone and UVA exposure. DNA damage produced by sparfloxacin and UVA in RPE cells could be remedied by the use of antioxidants, indicating a possible in vivo method for prevention or minimization of retinal damage in humans.

Revue / Journal Title

Cell biology and toxicology   ISSN 0742-2091   CODEN CBTOE2 

Source / Source

2000, vol. 16, n^o5, pp. 303-312 (34 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1984) (Revue)

Mots-clés anglais / English Keywords

Sparfloxacin ; Human ; In vitro ; Established cell line ; Epithelial cell ; Pigment cell ; Retina ; Eye ; Toxicity ; Toxic association ; Antibacterial agent ; Peroxides Hydrogen ; Ultraviolet irradiation ; UVA radiation ; Biological effect ; DNA ; Oxidative stress ; Free radical ; Fluoroquinolone derivatives ; Quinolone derivatives ; Antioxidant ; Molecular adduct ; Lesion ;

Mots-clés français / French Keywords

Sparfloxacine ; Homme ; In vitro ; Lignée cellulaire établie ; Cellule épithéliale ; Cellule pigmentaire ; Rétine ; Oeil ; Toxicité ; Association toxique ; Antibactérien ; Peroxyde Hydrogène ; Irradiation UV ; Rayonnement UVA ; Effet biologique ; DNA ; Stress oxydatif ; Radical libre ; Fluoroquinolone dérivé ; Quinolone dérivé ; Antioxydant ; Adduit moléculaire ; Lésion ;

Mots-clés espagnols / Spanish Keywords

Esparfloxacino ; Hombre ; In vitro ; Línea celular establecida ; Célula epitelial ; Célula pigmentaria ; Retina ; Ojo ; Toxicidad ; Asociación toxíca ; Antibacteriano ; Peróxido Hidrógeno ; Irradiación UV ; Radiación UVA ; Efecto biológico ; DNA ; Stress oxidativo ; Radical libre ; Fluoroquinolone derivado ; Quinolone derivado ; Antioxidante ; Producto adición molecular ; Lesión ;

Localisation / Location

INIST-CNRS, Cote INIST : 21552, 35400009403123.0040