Titre du document / Document title
Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni Transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses
Auteur(s) / Author(s)
CARY L. C.
(1) ;
GOEBEL M. ;
CORSARO B. G. ;
HWEI-GENE WANG ;
ROSEN E. ;
FRASER M. J. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Univ. Notre Dame, dep. biological sci., Notre Dame IN 46556, ETATS-UNIS
Résumé / Abstract
The transposable IFP2 element of Trichoplusia ni was originally isolated as a host DNA insertion in spontaneous FP mutants of Galleria mellonella or Autographa californica nuclear polyhedrosis viruses (NPVs). The termini of IFP2 insertions from five independently isolated FP mutants were sequenced. In all cases IFP2 is flanked by 13-bp terminal inverted repeats and has additional inverted repeats of 19bp in length located asymmetrically with respect to the ends of the element. Insertion of IFP2 into the viral genome always generated a duplication of the tetranucleotide target site, TTAA
Revue / Journal Title
Virology
ISSN 0042-6822
CODEN VIRLAX
Source / Source
1989, vol. 172, n
o1, pp. 156-169 (3 p.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, San Diego, CA, ETATS-UNIS
(1955)
(Revue)
Mots-clés anglais / English Keywords
Mutagenesis ;
Transposon ;
Inverted repeated sequence ;
Site ;
Integration ;
Molecular cloning ;
Nucleotide sequence ;
DNA ;
Molecular hybridization ;
Nuclear polyhedrosis virus ;
Trichoplusia ni ;
Nucleic acid ;
Primer extension analyis ;
Baculovirus ;
Baculoviridae ;
Virus ;
Noctuidae ;
Lepidoptera ;
Insecta ;
Arthropoda ;
Invertebrata ;
Mots-clés français / French Keywords
Mutagenèse ;
Transposon ;
Séquence répétée inversée ;
Site ;
Intégration ;
Clonage moléculaire ;
Séquence nucléotide ;
DNA ;
Hybridation moléculaire ;
Virus polyédrose nucléaire ;
Trichoplusia ni ;
Acide nucléique ;
Elément IFP2 ;
Méthode extension amorce ;
Baculovirus ;
Baculoviridae ;
Virus ;
Noctuidae ;
Lepidoptera ;
Insecta ;
Arthropoda ;
Invertebrata ;
Mots-clés espagnols / Spanish Keywords
Mutagénesis ;
Transposón ;
Secuencia repetida inversa ;
Sitio ;
Integración ;
Clonación molecular ;
Secuencia nucleótido ;
DNA ;
Hibridación molecular ;
Nuclear polyhedrosis virus ;
Trichoplusia ni ;
Acido nucleico ;
Baculovirus ;
Baculoviridae ;
Virus ;
Noctuidae ;
Lepidoptera ;
Insecta ;
Arthropoda ;
Invertebrata ;
Localisation / Location
INIST-CNRS, Cote INIST : 7801,
Nº notice refdoc (ud4) : 7373898