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Titre du document / Document title

Development of improved media for axenic cultivation of Acanthamoeba culbertsoni, Singh and Das 1970

Auteur(s) / Author(s)

SHUKLA O. P. (1) ; KAUL S. M. ; MEHLOTRA R. K. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Cent. drug res. inst., div. biochemistry, Lucknow 226 001, INDE

Résumé / Abstract

Several varieties of peptone supported growth of A. culbertsoni to different extents reaching a maximum cell density of 1-2×106/ml. A combination of proteose peptone with glucose, yeast extract and salts promoted excellent growth of A. culbertsoni with cell density reaching 1-2×107 cells/ml; tryptone and one of the indigenous peptones also yielded comparable growth when substituted for proteose peptone in this medium. Development of several media with or without glucose will aid in cultivation of A. culbertsoni, studies on its metabolism as well as screening of potential drugs

Revue / Journal Title

Indian journal of experimental biology   ISSN 0019-5189   CODEN IJEBA6 

Source / Source

1989, vol. 900027, no9, pp. 785-791 (41 ref.)

Langue / Language

Anglais

Editeur / Publisher

Council of Scientific & Industrial Research, New Delhi, INDE  (1963) (Revue)

Mots-clés anglais / English Keywords

Culture medium ; Microorganism culture ; Microorganism growth ; Substrate ; Optimization ; Cell density ; Nutrient ; Energy source ; Carbohydrate ; Acanthamoeba culbertsoni ; Rhizoflagellata ; Protozoa ;

Mots-clés français / French Keywords

Milieu culture ; Culture microorganisme ; Multiplication microorganisme ; Substrat ; Optimisation ; Densité cellulaire ; Nutriment ; Source énergie ; Glucide ; Acanthamoeba culbertsoni ; Peptone ; Rhizoflagellata ; Protozoa ;

Mots-clés espagnols / Spanish Keywords

Medio cultivo ; Cultivo microorganismo ; Multiplicación microorganismo ; Substrato ; Optimización ; Densidad celular ; Nutriente ; Fuente energética ; Glúcido ; Acanthamoeba culbertsoni ; Rhizoflagellata ; Protozoa ;

Localisation / Location

INIST-CNRS, Cote INIST : 2364 C, 35400000774092.0020

Nº notice refdoc (ud4) : 6926992

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