Titre du document / Document title
Nickel induces a signature mutation for oxygen free radical damage
Auteur(s) / Author(s)
TKESHELASHVILI L. K. ;
REID T. M. ;
MCBRIDE T. J. ;
LOEB L. A. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
Univ. Washington, Joseph Gottstein memorial cancer res. lab., dep. pathology, Seattle WA 98195, ETATS-UNIS
Résumé / Abstract
We have determined the specificity of mutations produced by nickel(II), a known human carcinogen, in a forward mutation assay and also used a sensitive reversion assay to show that Ni(II), like iron and copper, can produce tandem double CC→TT mutatinns, a hallmark of damage to DNA by either UV irradiation or oxygen free radicals. A reduction in mutation frequencies by the addition of oxygen radical scavengers also supports the involvement of reactive oxygen species in DNA damage andmutagenesis by Ni(II). Mutagenesis by Ni(II) is enhanced by the addition of both hydrogen peroxide and a topeptide glycyl-glycyl-L-histidine. The enhancement of mutagenesis of Ni(II) by the tripeptide indicates that these complexes could serve to localize Ni(II) in nuclei and mediate DNA damage and mutagenesis via the generation of short-lived oxygen free radicals
Revue / Journal Title
Cancer research
ISSN 0008-5472
CODEN CNREA8
Source / Source
1993, vol. 53, n
o18, pp. 4172-4174 (21 ref.)
Langue / Language
Anglais
Editeur / Publisher
American Association for Cancer Research, Philadelphia, PA, ETATS-UNIS
(1941)
(Revue)
Mots-clés anglais / English Keywords
Nickel ;
Toxicity ;
Carcinogen ;
Mutagen ;
Mechanism of action ;
Free radical ;
Oxygen ;
Biological fixation ;
Nuclear protein ;
In vitro ;
Heavy metal ;
Mots-clés français / French Keywords
Nickel ;
Toxicité ;
Carcinogène ;
Mutagène ;
Mécanisme action ;
Radical libre ;
Oxygène ;
Fixation biologique ;
Protéine nucléaire ;
In vitro ;
Métal lourd ;
Oxygène actif ;
Mots-clés espagnols / Spanish Keywords
Niquel ;
Toxicidad ;
Carcinógeno ;
Mutágeno ;
Mecanismo acción ;
Radical libre ;
Oxígeno ;
Fijación biológica ;
Proteína nuclearia ;
In vitro ;
Metal pesado ;
Localisation / Location
INIST-CNRS, Cote INIST : 5088, 35400004797925.0110
Nº notice refdoc (ud4) : 4896848
