Titre du document / Document title

Isolation and culture of highly enriched populations of Leydig cells from guinea-pig (Caviaporcellus) testes

Auteur(s) / Author(s)

KUKUCKA M. A. ; MISRA H. P. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

Virginia polytech. inst., Virginia-Maryland regional coll. veterinary medicine, dep. biomedical sci., Blacksburg VA 24061-0442, ETATS-UNIS

Résumé / Abstract

Leydig cells were isolated from adult male guinea-pig testes using a multi-step procedure involving enzymatic dissociation and Percollgradient centrifugation. The following description is the first account of a successful isolation of adolescent guinea-pig Leydig cells. The enriched Leydig-cell preparation routinely isolated from six intact testicles yielded approximately 5.0 × 10⁶ ± 0. 7 × 10⁶ (± SEM) Leydig cells with a viability of 98.0 ± 0.4% as determined using the trypan-blue exclusion method. The purity of the isolated cell population as assessed by 3β-hydroxysteroid dehydrogenase (3β-HSD) staining averaged 82.5 ± 0.8%. Under light microscopy, guinea-pig Leydig cells were polyhedral in shape with a large prominent nucleus and a distinct nucleolus

Revue / Journal Title

Andrologia   ISSN 0303-4569   CODEN ANDRDQ 

Source / Source

1994, vol. 26, n^o4, pp. 217-224 (27 ref.)

Langue / Language

Anglais

Editeur / Publisher

Blackwell, Berlin, ALLEMAGNE  (1969) (Revue)

Mots-clés anglais / English Keywords

Leydig cell ; Cell culture ; Isolation ; Steroidogenesis ; Culture medium ; Ultrastructure ; Guinea pig ; Male ; Testicle ; Male genital system ; Rodentia ; Mammalia ; Vertebrata ;

Mots-clés français / French Keywords

Cellule Leydig ; Culture cellulaire ; Isolement ; Stéroïdogenèse ; Milieu culture ; Ultrastructure ; Cobaye ; Mâle ; Testicule ; Appareil génital mâle ; Rodentia ; Mammalia ; Vertebrata ;

Mots-clés espagnols / Spanish Keywords

Célula Leydig ; Cultivo celular ; Aislamiento ; Esteroidogénesis ; Medio cultivo ; Ultraestructura ; Cobayo ; Macho ; Testículo ; Aparato genital macho ; Rodentia ; Mammalia ; Vertebrata ;

Localisation / Location

INIST-CNRS, Cote INIST : 17487, 35400004686706.0030