Titre du document / Document title
Fate of a mutant macrochloroplast in somatic hybrids
Auteur(s) / Author(s)
ROTHER S. ;
HADELER B. ;
ORSINI J. M. ;
ABEL W. O. ;
RESKI R. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
Inst. Allgemeine Botanik, 22609 Hamburg, ALLEMAGNE
Résumé / Abstract
Protoplast isolation from a moss, Physcomitrella patens (Hedw.)B.S.G., was optimized regarding preculture of plants, digestion procedure and isolation protocol. PEG-mediated protoplast fusion in batch experiments resulted in at least 29 % fusion products. All of these appeared to be one-to-one fusions. In about 50% of the fusants nuclei fused within 90 minutes as well. Protoplast fusion with wild-type and chloroplast mutant PC22 was performed. Fusion products harbouring wild-type plastids as well as a giant mutant chloroplast were transferred to feeder-cultures in millicells and fate of the macrochloroplast was observed
Revue / Journal Title
Journal of plant physiology
ISSN
0176-1617
CODEN JPPHEY
Source / Source
1994, vol. 143, n
o1, pp. 72-77 (28 ref.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, Munich, ALLEMAGNE
(1984)
(Revue)
Mots-clés anglais / English Keywords
;
;
;
;
;
;
;
;
;
Mots-clés français / French Keywords
;
;
;
;
;
;
;
;
;
;
Mots-clés espagnols / Spanish Keywords
;
;
;
;
;
;
;
;
;
Localisation / Location
INIST-CNRS, Cote INIST : 922, 35400004908936.0110
Nº notice refdoc (ud4) : 4143174
