Titre du document / Document title
A radioimmmunoassay for the quantification of human ubiquitin in biological fluids : application to parasitic and allergic diseases
Auteur(s) / Author(s)
ASSEMAN C. ;
PANCRE V. ;
DELANOYE A. ;
CAPRON A. ;
AURIAULT C. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
Inst. Pasteur, cent. immunologie biologie parasitaire, 59019 Lille, FRANCE
Résumé / Abstract
Purified ubiquitin has been shown to share similar biological and physicochemical properties with a previously characterized lymphokine, platelet activity suppressive lymphokine (PASL). This lymphokine, which inhibits the cytotoxic function of activated platelets, is produced during schistosomiasis and Hymenoptera venom hypersensitivity (HVH). We have developed a radioimmunoassay specific for ubiquitin, in order to determine the ubiquitin levels in human sera and plasma from patients with these pathologies. The working range of the assay was between 60 and 500 ng/ml, and the sensitivity was 8-10 ng/ml
Revue / Journal Title
Journal of immunological methods
ISSN 0022-1759
CODEN JIMMBG
Source / Source
1994, vol. 173, n
o1, pp. 93-101 (1 p.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, Amsterdam, PAYS-BAS
(1971)
(Revue)
Mots-clés anglais / English Keywords
Human ;
Biological fluid ;
Quantitative analysis ;
Ubiquitin ;
Radioimmunoassay ;
Pathogenesis ;
Diagnosis ;
Venom ;
Hymenoptera ;
Hypersensitivity ;
Schistosomiasis ;
Insecta ;
Arthropoda ;
Invertebrata ;
Trematod disease ;
Helminthiasis ;
Parasitosis ;
Infection ;
Proteins ;
Mots-clés français / French Keywords
Homme ;
Liquide biologique ;
Analyse quantitative ;
Ubiquitine ;
Méthode radioimmunologique ;
Pathogénie ;
Diagnostic ;
Venin ;
Hymenoptera ;
Hypersensibilité ;
Schistosomiase ;
Insecta ;
Arthropoda ;
Invertebrata ;
Trématodose ;
Helminthiase ;
Parasitose ;
Infection ;
Protéine ;
Mots-clés espagnols / Spanish Keywords
Hombre ;
Líquido biológico ;
Análisis cuantitativo ;
Ubiquitina ;
Método radioinmunológico ;
Patogenia ;
Diagnóstico ;
Veneno ;
Hymenoptera ;
Hipersensibilidad ;
Esquistosomiasis ;
Insecta ;
Arthropoda ;
Invertebrata ;
Trematodosis ;
Helmintiasis ;
Parasitosis ;
Infección ;
Proteína ;
Localisation / Location
INIST-CNRS, Cote INIST : 15654, 35400004019577.0120
Nº notice refdoc (ud4) : 4138043
