Titre du document / Document title

Positive and negative regulatory elements are involved in transcriptional control of the rat glucokinase gene in the insulin producing cell line HIT M2.2.2

Auteur(s) / Author(s)

LEIBIGER I. B. ; WALTHER R. ; PETT U. ; LEIBIGER B. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

Ernst-Moritz-Arndt-Univ. Greifswald, Medizinische Fak., Inst. Biochemie, 17489 Greifswald, ALLEMAGNE

Résumé / Abstract

Nested deletion mutants of the 5' flanking region of the β-cell transcription unit of the rat glucokinase gene (rβGK) were fused to the CAT-reporter gene. Transient expression studies in HIT M2.2. 2 and BHK21 cells revealed a distal (upstream of -359) and a proximal promoter region (between -278/-49) harbouring positive and negative regulatory elements. DNaseI footprinting revealed three protected areas between nucleotides -190 and -60. DNA-elements playing a crucial role in transcriptional control of the insulin genes (IEB- and CT-motifs) have been detected within the proximal promoter region and contribute to β-cell specific gene regulation. 3' deletion analysis revealed that DNA-elements located downstream from transcription initiation sites (up to +123) contribute to transcriptional regulation

Revue / Journal Title

FEBS letters   ISSN 0014-5793   CODEN FEBLAL 

Source / Source

1994, vol. 337, n^o2, pp. 161-166 (34 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1968) (Revue)

Mots-clés anglais / English Keywords

Rat ; Gene ; Mutation ; Glucokinase ; Transcription promoter ; Cell line ; Reporter gene ; Deletion ; Transcription initiation ; Rodentia ; Mammalia ; Vertebrata ; Transferases ; Enzyme ;

Mots-clés français / French Keywords

Rat ; Gène ; Mutation ; Glucokinase ; Promoteur transcription ; Lignée cellulaire ; Gène indicateur ; Délétion ; Initiation transcription ; Rodentia ; Mammalia ; Vertebrata ; Transferases ; Enzyme ;

Mots-clés espagnols / Spanish Keywords

Rata ; Gen ; Mutación ; Glucokinase ; Promotor transcripción ; Línea celular ; Gen marcador ; Deleción ; Iniciación transcripción ; Rodentia ; Mammalia ; Vertebrata ; Transferases ; Enzima ;

Localisation / Location

INIST-CNRS, Cote INIST : 13934, 35400002473966.0090