Titre du document / Document title
The antioxidant defense system of isolated guinea pig Leydig cells
Auteur(s) / Author(s)
KUKUCKA M. A. ;
MISRA H. P. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
Virginia polytech. inst. State univ., Virginia-Maryland regional coll. veterinary medicine, dep. biomedical sci., Blacksburg VA 24061, ETATS-UNIS
Résumé / Abstract
Utilization of highly enriched preparations of steroidogenic Leydig cells have proven invaluable for studying the direct effects of various hormones and agents on Leydig cell function in vitro. However, recent work indicates that isolated Leydig cells are often subjected to oxygen (O
2) toxicity when cultured at ambient (19%) oxygen concentrations. Because intracellular antioxidants play an important role in protecting cells against oxygen toxicity, we have investigated the intracellular antioxidant defense system of isolated Leydig cells. The cellular levels of several antioxidants including catalase, glucose-6-phosphate dehydrogenase (G-6-PDH), superoxide dismutase (SOD) of the Cu/Zn & Mn variety, glutathione peroxidase, glutathione reductase and total glutathione were quantitated using enriched populations of Leydig cells isolated from adult male guinea pig testes
Revue / Journal Title
Molecular and cellular biochemistry
ISSN 0300-8177
Source / Source
1993, vol. 126, n
o1, pp. 1-7 (34 ref.)
Langue / Language
Anglais
Editeur / Publisher
Springer, Dordrecht, PAYS-BAS
(1973)
(Revue)
Mots-clés anglais / English Keywords
Guinea pig ;
Leydig cell ;
Antioxidant ;
Cytoprotector ;
Enzymatic activity ;
Superoxide dismutase ;
Glutathione peroxidase ;
Catalase ;
Hydrogenperoxides ;
In vitro ;
Rodentia ;
Mammalia ;
Vertebrata ;
Oxidoreductases ;
Enzyme ;
Peroxidases ;
Mots-clés français / French Keywords
Cobaye ;
Cellule Leydig ;
Antioxydant ;
Cytoprotecteur ;
Activité enzymatique ;
Superoxide dismutase ;
Glutathione peroxidase ;
Catalase ;
Hydrogénoperoxyde ;
In vitro ;
Rodentia ;
Mammalia ;
Vertebrata ;
Oxidoreductases ;
Enzyme ;
Peroxidases ;
Mots-clés espagnols / Spanish Keywords
Cobayo ;
Célula Leydig ;
Antioxidante ;
Citoprotector ;
Actividad enzimática ;
Superoxide dismutase ;
Glutathione peroxidase ;
Catalase ;
Hidrogenoperóxido ;
In vitro ;
Rodentia ;
Mammalia ;
Vertebrata ;
Oxidoreductases ;
Enzima ;
Peroxidases ;
Localisation / Location
INIST-CNRS, Cote INIST : 16246, 35400002646108.0010
Nº notice refdoc (ud4) : 3826630
