Titre du document / Document title

The influence of insulin, β-estradiol, dexamethasone and thyroid hormone on the secretion of coagulant and anticoagulant proteins by HepG2 cells

Auteur(s) / Author(s)

NIESSEN R. W. L. M. ⁽¹⁾ ; PFAFFENDORF B. A. ⁽¹⁾ ; STURK A. ; LAMPING R. J. ⁽¹⁾ ; SCHAAP M. C. L. ; HACK C. E. ; PETERS M. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Univ. Amsterdam, acad. medical cent., cent. hemostasis thrombosis atherosclerosis inflammation res., 1100 DE Amsterdam, PAYS-BAS

Résumé / Abstract

As a basis for regulatory studies on the influence of hormones on (anti)coagulant protein production by hepatocytes, we examined the amounts of the plasma proteins antithrombin III (AT III), protein C, protein S, factor II. factor X, fibrinogen, and prealbumin produced by the hepatoma cell line HepG2, into the culture medium, in the absence and presence of insulin. β-estradiol, dexamethasone and thyroid hormone. Without hormones these cells produced large amounts of fibrinogen (2,452 ± 501 ng/mg cell protein), AT III (447 + 16 ng/mg cell protein) and factor 11 (464 ± 31 ng/mg cell protein) and only small amounts of protein C (50 ± 7 ng/mg cell protein) and factor X (55 ± 5 ng/mg cell protein). Thyroid hormone had a slight but significant effect on the enrichment in the culture medium of the anticoagulant protein AT III (1.34-fold) but not on protein C (0.96-fold) and protein S (0.91-fold). This hormone also significantly increased the amounts of the coagulant proteins factor II (1.28-fold), factor X (1.45-fold) and fibrinogen (2.17-fold). Insulin had an overall stimulating effect on the amounts of all the proteins that were investigated. Neither dexamethasone nor β-estradiol administration did substantially change the amounts of these proteins. We conclude that the HepG2 cell is a useful tool to study the hormonal regulation of the production of (anti)coagulant proteins. We studied the overall process of protein production, i.e., the amounts of proteins produced into the culture medium. Detailed studies have to be performed to establish the specific hormonal effects on the underlying processes. e.g., transcription, translation, cellular processing and transport, and secretion.

Revue / Journal Title

Thrombosis and haemostasis   ISSN 0340-6245   CODEN THHADQ 

Source / Source

1995, vol. 74, n^o2, pp. 686-692 (34 ref.)

Langue / Language

Anglais

Editeur / Publisher

Schattauer, Stuttgart, ALLEMAGNE  (1976) (Revue)

Mots-clés anglais / English Keywords

Hepatocyte ; Liver ; Established cell line ; Insulin ; Anticoagulant ; Endogenous ; 17β-Estradiol ; Thyroid hormone ; Secretion ; Dexamethasone ; Prothrombin ; Factor X ; Fibrinogen ; Antithrombin III ; Protein C ; Vitronectin ; Human ; Corticosteroid ; Blood coagulation ; Coagulation factor ; Protein hormone ; Pancreatic hormone ; Estrogen ; Ovarian hormone ; Sex steroid hormone ;

Mots-clés français / French Keywords

Hépatocyte ; Foie ; Lignée cellulaire établie ; Insuline ; Anticoagulant ; Endogène ; 17β-Oestradiol ; Hormone thyroïdienne ; Sécrétion ; Dexaméthasone ; Prothrombine ; Facteur Stuart ; Fibrinogène ; Antithrombine III ; Protéine C ; Vitronectine ; Homme ; Corticostéroïde ; Lignée HepG2 ; Coagulation sanguine ; Facteur coagulation ; Hormone protéine ; Hormone pancréatique ; Oestrogène ; Hormone ovarienne ; Hormone stéroïde sexuelle ;

Mots-clés espagnols / Spanish Keywords

Hepatocito ; Hígado ; Línea celular establecida ; Insulina ; Anticoagulante ; Endógeno ; 17β-Estradiol ; Hormona tiroidea ; Secreción ; Protrombina ; Factor Stuart ; Fibrinógeno ; Antitrombina III ; Proteína C ; Vitronectina ; Hombre ; Corticoesteroide ; Coagulación sanguínea ; Factor coagulación ; Hormona proteina ; Hormona pancreática ; Estrógeno ; Hormona ovárica ; Hormona esteroide sexual ;

Localisation / Location

INIST-CNRS, Cote INIST : 10255, 35400005371654.0210