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Titre du document / Document title

A new fully automated system for the morphometric analysis of human sperm leads

Auteur(s) / Author(s)

GARRETT C. ; BAKER H. W. G. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

Royal womens hosp., univ. Melbourne dep. obstetrics gynecology, reproductive biology unit, Melbourne Victoria, AUSTRALIE

Résumé / Abstract

Objective: To develop a fully automated image analysis system to provide objective, quantitative, and reproducible assessment of the head morphology of human spermatozoa. Design: Shorr-stained smears of washed sperm were imaged with a microscope (×100) and black and white video camera and digitized for computer analysis. Automatically selected sperm head images are analyzed in terms of 32 parameters that reflect size, shape, and staining heterogeneity. Unique features of the analysis include extraction of a 40% density contour in addition to the usual boundary contour and parameterization of optical density profiles. The morphometry of a semen sample is summarized by evaluation of the sample mean and standard deviation for each morphometric parameter. In addition, each analyzed sperm in a sample is assessed for conformity to a reference morphometric data set. Results: The system filters seminal debris, superimposed cells, and lysed sperm, with an average sperm recognition error of 2.3% and contour interpretation error of 1.7%. The average proportion of variance attributable to slide preparation and analysis methods (2.6%) was low relative to that between infertility patients (12.4%). The relatively slow assessment times would be improved substantially with upgraded hardware. Conclusion: The precision and sensitivity offered by the detailed image analysis of this system provides a powerful tool for morphometric semen analysis

Revue / Journal Title

Fertility and sterility    ISSN  0015-0282   CODEN FESTAS 

Source / Source

1995, vol. 63, no6, pp. 1306-1317 (21 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, New York, NY, ETATS-UNIS  (1950) (Revue)

Mots-clés anglais / English Keywords

Spermatozoa

;

Semen

;

Morphology

;

Morphometry

;

Exploration

;

Image analysis

;

Computer aided analysis

;

Male sterility

;

Semen disorders

;

Technique

;

Accuracy

;

Sensitivity

;

Human

;

Male

;

Germinal cell

;

Male genital diseases

;

Mots-clés français / French Keywords

Spermatozoïde

;

Sperme

;

Morphologie

;

Morphométrie

;

Exploration

;

Analyse image

;

Analyse assistée

;

Stérilité mâle

;

Sperme pathologie

;

Technique

;

Précision

;

Sensibilité

;

Homme

;

Mâle

;

Cellule germinale

;

Appareil génital mâle pathologie

;

Mots-clés espagnols / Spanish Keywords

Espermatozoide

;

Esperma

;

Morfología

;

Morfometría

;

Exploración

;

Análisis imagen

;

Análisis asistido

;

Esterilidad masculina

;

Esperma patología

;

Técnica

;

Precisión

;

Sensibilidad

;

Hombre

;

Macho

;

Célula germinal

;

Aparato genital macho patología

;

Localisation / Location

INIST-CNRS, Cote INIST : 4120, 35400005687026.0280

Nº notice refdoc (ud4) : 3518124



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