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Titre du document / Document title

Inhibition of human plasma and serum butyrylcholinesterase (EC 3.1.1.8) by α-chaconine and α-solanine

Auteur(s) / Author(s)

NIGG H. N. (1) ; RAMOS L. E. (1) ; GRAHAM E. M. (1) ; STERLING J. (1) ; BROWN S. (1) ; CORNELL J. A. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, Lake Alfred, Florida 33850, ETATS-UNIS

Résumé / Abstract

The purpose of these experiments was to determine the reversibility of α-chaconine and α-solanine inhibition of human plasma butyrylcholinesterase (BuChE). For the substrate α-naphthylacetate, optimal assay conditions were 0.50 M sodium phosphate buffer and a substrate concentration of 3-5 x 10-4 M : Dibucaine (1 x 10-5 M) indicated the usual phenotype for all subjects ; α-chaconine and α-solanine at 2.88 x 10-6 M inhibited BuChE about 70 and 50%, respectively. One- and 24-hr incubations at 1 x 10-5 M with α-chaconine, α-solanine, paraoxon, eserine, and ethanol yielded reversible inhibition with dilution except for paraoxon. Twenty-four-hour dialyses of incubations showed no inhibition except for paraoxon. PAGE enzyme activity gels of 1- and 24-hr incubations also showed no inhibition except for paraoxon. α-Chaconine and α-solanine are reversible inhibitors of human butyrylcholinesterase. At estimated tissue levels, α-chaconine, α-solanine, and solanidine inhibited BuChE 10-86%. In assays which combined α-chaconine, α-solanine, and solanidine, inhibition of BuChE was less than additive. No inhibition of albumin α-naphthylacetate esterase (an arylesterase) was noted with any inhibitor. The importance of these data to adverse toxicological effects of potato alkaloids is discussed

Revue / Journal Title

Fundamental and applied toxicology    ISSN  0272-0590   CODEN FAATDF 

Source / Source

1996, vol. 33, no2, pp. 272-281 (2 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Academic Press, San Diego, CA, ETATS-UNIS  (1981-1997) (Revue)

Mots-clés anglais / English Keywords

Toxicity

;

Solanine

;

Alkaloid

;

Glycoside

;

Potato

;

Enzyme inhibitor

;

Cholinesterase

;

Blood plasma

;

Serum

;

Human

;

Reversibility

;

Carboxylic ester hydrolases

;

Esterases

;

Hydrolases

;

Enzyme

;

Mots-clés français / French Keywords

Toxicité

;

Solanine

;

Alcaloïde

;

Glycoside

;

Pomme de terre

;

Inhibiteur enzyme

;

Cholinesterase

;

Plasma sanguin

;

Sérum

;

Homme

;

Réversibilité

;

Chaconine

;

Carboxylic ester hydrolases

;

Esterases

;

Hydrolases

;

Enzyme

;

Mots-clés espagnols / Spanish Keywords

Toxicidad

;

Solanina

;

Alcaloide

;

Glicósido

;

Patata

;

Inhibidor enzima

;

Cholinesterase

;

Plasma sanguíneo

;

Suero

;

Hombre

;

Reversibilidad

;

Carboxylic ester hydrolases

;

Esterases

;

Hydrolases

;

Enzima

;

Localisation / Location

INIST-CNRS, Cote INIST : 19134, 35400006687082.0130

Nº notice refdoc (ud4) : 3255160



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