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Titre du document / Document title

Effects of auxins and cytokinins on tissue culture of Grateloupia dichotoma (Gigartinales, Rhodophyta)

Auteur(s) / Author(s)

YOKOYA N. S. (1) ; HANDRO W. (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Institute of Botany, Secretary of Environment, State of São Paulo, C.P. 4005, 01061-970, São Paulo, BRESIL
(2) Plant Cell Biology Laboratory, Institute of Biosciences, University of São Paulo, C.P. 11461, 05422-970, São Paulo, BRESIL

Résumé / Abstract

The role played by plant growth regulators in algae is poorly known. In order to increase the knowledge about the function of auxins and cytokinins in seaweeds, explants such as apical and intercalary segments and callus-like structures (CLS) of Grateloupia dichotoma were cultured in semi-solid or liquid artificial media ASP 12-NTA. Two auxins, indole-3-acetic acid (IAA) and 2,4-dichlorophenoxyacetic acid (2,4-D), and one cytokinin, 6-benzylaminopurine (BA), at concentrations of 0.5 and 5.0 mg l-1 were tested. Moreover, IAA and BA were tested together at concentrations of 1 :5 and 5 :1 mg l-1. All treatments promoted the growth of CLS in intercalary segments ; CLS from apical segments were significantly higher in treatments with 2,4-D or IAA :BA (1 :5 mg l-1). The morphogenetic responses for auxins and BA were opposite, auxins inhibited while BA promoted the formation of lateral branches ; however, auxins promoted the elongation of such branches. The process of plant regeneration observed on CLS was stimulated significantly by treatment with high concentration of BA or IAA :BA (1 :5 mg l-1) in semi-solid and liquid media. The growth of upright axes was stimulated significantly by treatment with 2,4-D in semi-solid medium, and IAA :BA (1 :5 mg l-1) in liquid medium. These results show the importance that plant growth regulators could have in the control of growth, morphogenetic processes and micropropagation in red algae.

Revue / Journal Title

Hydrobiologia    ISSN  0018-8158   CODEN HYDRB8 

Source / Source

Congrès
International Seaweed Symposium No15, Valdivia , CHILI (01/1995)
1996, vol. 326-27 (574 p.)  (32 ref.), pp. 393-400

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1948) (Revue)

Mots-clés anglais / English Keywords

Cytokinin

;

Auxin

;

Callus

;

Tissue culture

;

Vegetative propagation

;

Regeneration

;

Growth

;

Morphogenesis

;

In vitro

;

Adenine(N6-benzyl)

;

2,4-D

;

Seaweed

;

Indoleacetic acid

;

Purine derivatives

;

Phytohormone

;

Algal culture

;

Rhodophycophyta

;

Algae

;

Thallophyta

;

Plant growth substance

;

Marine plant

;

Mots-clés français / French Keywords

Cytokinine

;

Auxine

;

Cal

;

Culture tissu

;

Multiplication végétative

;

Régénération

;

Croissance

;

Morphogenèse

;

In vitro

;

Adénine(N6-benzyl)

;

2,4-D

;

Indole-3-acétique acide

;

Grateloupia dichotoma

;

Goémon

;

Indoleacétique acide

;

Purine dérivé

;

Phytohormone

;

Algoculture

;

Rhodophycophyta

;

Algae

;

Thallophyta

;

Substance croissance végétal

;

Plante marine

;

Mots-clés espagnols / Spanish Keywords

Citoquinina

;

Auxina

;

Callo

;

Cultivo tejido

;

Multiplicación vegetativa

;

Regeneración

;

Crecimiento

;

Morfogénesis

;

In vitro

;

Adenina(N6-benzil)

;

2,4-D

;

Indoloacetico ácido

;

Purina derivado

;

Fitohormona

;

Algacultura

;

Rhodophycophyta

;

Algae

;

Thallophyta

;

Substancia crecimiento vegetal

;

Planta marina

;

Localisation / Location

INIST-CNRS, Cote INIST : 5329, 35400006419890.0550

Nº notice refdoc (ud4) : 3245542



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