Titre du document / Document title

Expression and localization of inhibin/activin and activin receptors in GH3 cells, a rat pituitary adenocarcinoma cell line

Auteur(s) / Author(s)

YING C. ⁽¹⁾ ; ZHANG Z. ⁽¹⁾ ; HUANG G. ⁽¹⁾ ; LI S. Q. ⁽¹⁾ ; YING S. Y. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Cell and Neurobiology, University of Southern California School of Medicine, Los Angeles, California, ETATS-UNIS

Résumé / Abstract

Inhibins and activins are members of the transforming growth factor β (TGFB) superfamily. Since TGFβ has been shown to be a potent proliferation-inhibiting agent for the pituitary adenocarcinoma cell line, GH3, we determined whether this cell line (a) transcribes mRNAs coding for inhibin/activin subunits (α and βA) and activin receptors I, II, and IIB ; and (b) produces inhibin and/or activin proteins. Messenger RNAs for the α- and βA-subunits of inhibin and activin receptors I, II, and IIB in GH3 cells were detected and localized using the reverse transcription-polymerase chain reaction (RT-PCR) analysis and in situ hybridization, respectively. The identity of the RT-PCR products was confirmed by DNA sequencing of PCR products. Immunocytochemically, inhibin and activin were localized in these cells. Our findings indicate that messenger RNAs encoding inhibin α- and βA-subunits and activin receptors I, II, and IIB were expressed, and inhibin/activin proteins were produced, by GH3 cells, imply that these gonadal growth factors may have paracrine/autocrine functions in rat pituitary adenocarcinoma. Further, these observation suggest that these growth factors may be involved in regulating the growth and differentiational of rat pituitary adenocarcinoma cells.

Revue / Journal Title

Journal of endocrinological investigation   ISSN 0391-4097   CODEN JEIND7 

Source / Source

1996, vol. 19, n^o1, pp. 6-11 (33 ref.)

Langue / Language

Anglais

Editeur / Publisher

Kurtis, Milano, ITALIE  (1978) (Revue)

Mots-clés anglais / English Keywords

Adenocarcinoma ; Pituitary gland ; Transforming growth factor β ; Inhibin ; Activin ; Molecular biology ; Immunocytochemistry ; Animal model ; Animal ; Rat ; Rodentia ; Mammalia ; Vertebrata ; Malignant tumor ; Pituitary diseases ; Endocrinopathy ; Peptide hormone ; Pathology ;

Mots-clés français / French Keywords

Adénocarcinome ; Hypophyse ; Facteur croissance transformant β ; Inhibine ; Activine ; Biologie moléculaire ; Immunocytochimie ; Modèle animal ; Animal ; Rat ; Rodentia ; Mammalia ; Vertebrata ; Tumeur maligne ; Hypophyse pathologie ; Endocrinopathie ; Hormone peptide ; Anatomopathologie ;

Mots-clés espagnols / Spanish Keywords

Adenocarcinoma ; Hipófisis ; Factor crecimiento transformante β ; Inhibina ; Activina ; Biología molecular ; Inmunocitoquímica ; Modelo animal ; Animal ; Rata ; Rodentia ; Mammalia ; Vertebrata ; Tumor maligno ; Hipófisis patología ; Endocrinopatía ; Hormona péptido ; Anatomía patológica ;

Localisation / Location

INIST-CNRS, Cote INIST : 18833, 35400005311601.0020