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Titre du document / Document title

A sensitive sperm-motility test for the assessment of cytotoxic effect of pesticides

Auteur(s) / Author(s)

YOUSEF M. I. (1) ; BERTHEUSSEN K. ; IBRAHIM H. Z. (1) ; HELMI S. (1) ; SEEHY M. A. ; SALEM M. H. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Alexandria univ., inst. graduate studies res., dep. environmental studies, EGYPTE

Résumé / Abstract

A sensitive sperm-motility test for the evaluation of cytotoxic effects of carbofuran and glyphosate in a defined protein-free culture medium is described. The sperm motility was compared to that obtained with a protein-containing medium. The use of protein-free medium considerably increased the sensitivity of sperm cells from rabbit and human to the toxic effects of the pesticide. The respective IC50 values (the concentration needed to cause 50% inhibition of sperm motility) in protein-free medium of carbofuran and glyphosate were 321 and 48.2 μM with human sperm, and 116 and 23.5 μM with rabbit sperm. Whereas, the corresponding values in protein-containing medium were 920 and 740 μM, and 910 and 500 μM with human and rabbit sperm, respectively. Our results show that testing human and rabbit sperm in protein-free medium proves to be a more sensitive method than that in protein-containing medium. Additionally, the use of rabbit sperm is a more sensitive test system than human sperm. This study suggests that the rabbit sperm test appears to have a potential for the assessment of toxicity on human reproduction.

Revue / Journal Title

Journal of environmental science and health. Part B. Pesticides, food contaminants, and agricultural wastes    ISSN  0360-1234   CODEN JPFCD2 

Source / Source

1996, vol. 31, no1, pp. 99-115 (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Taylor & Francis, Philadelphia, PA, ETATS-UNIS  (1976) (Revue)

Mots-clés anglais / English Keywords

Cytotoxicity

;

Semen

;

Rabbit

;

Human

;

Motility

;

Inhibition

;

Culture medium

;

Proteins

;

Bioassay

;

Oryctolagus cuniculus

;

Glyphosate

;

Carbofuran

;

Lagomorpha

;

Mammalia

;

Vertebrata

;

Toxicity

;

Pesticides

;

Ecotoxicology

;

Aminoacid

;

Herbicide

;

Carbamate

;

Insecticide

;

Mots-clés français / French Keywords

Cytotoxicité

;

Sperme

;

Lapin

;

Homme

;

Motilité

;

Inhibition

;

Milieu culture

;

Protéine

;

Essai biologique

;

Oryctolagus cuniculus

;

Glyphosate

;

Carbofuran

;

Lagomorpha

;

Mammalia

;

Vertebrata

;

Toxicité

;

Pesticide

;

Ecotoxicologie

;

Aminoacide

;

Herbicide

;

Carbamate

;

Insecticide

;

Mots-clés espagnols / Spanish Keywords

Citotoxicidad

;

Esperma

;

Conejo

;

Hombre

;

Motilidad

;

Inhibición

;

Medio cultivo

;

Proteína

;

Prueba biológica

;

Oryctolagus cuniculus

;

Glyphosate

;

Carbofurano

;

Lagomorpha

;

Mammalia

;

Vertebrata

;

Toxicidad

;

Plaguicida

;

Ecotoxicología

;

Aminoácido

;

Herbicida

;

Carbamato

;

Insecticida

;

Localisation / Location

INIST-CNRS, Cote INIST : 15710 B, 35400005279790.0060

Nº notice refdoc (ud4) : 2993485



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