Titre du document / Document title

Immunopathologic study of the conjunctiva in patients with Behçet disease

Auteur(s) / Author(s)

TUGAL-TUTKUN I. ⁽¹⁾ ; URGANCIOGLU M. ⁽¹⁾ ; FOSTER C. S. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Istanbul univ., Istanbul fac. medicine, ocular immunology uveitis serv., Istanbul, TURQUIE

Résumé / Abstract

Purpose : Even though conjunctiva is not primarily involved in patients with uveitis due to Behçet disease, it may reflect the immunopathologic process when inflammation is induced by biopsy of conjunctiva, a phenomenon similar to the induced inflammation at skin pathergy sites. Methods : Conjunctival biopsy specimens obtained 48 hours after a 2-mm biopsy of the epibulbar conjunctiva in 26 Turkish patients with inactive ocular Behçet disease and 9 Turkish patients with inactive idiopathic uveitis were studied by immunoperoxidase using a panel of monoclonal antibodies : anti-CD1, -CD3, -CD4, -CD5, -CD14, -CD22, -CD25, and -CD67, HLA-DR, E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1). Results : Immunopathology of the conjunctival specimens obtained at the time of first biopsy was not significantly different between the Behçet disease and the idiopathic uveitis groups. The second-biopsy specimens of the patients with Behçet disease showed significantly greater numbers of T cells (CD3+, CD4+) and granulocytes (CD67+) as well as HLA-DR+ and ICAM-1+ cells in the substantia propria. Vascular endothelium of the conjunctiva in a patient with Behçet disease had significantly more pronounced expression of the adhesion molecules, E-selectin, and ICAM-1. None of the conjunctival specimens in either group showed VCAM-1 positivity. Conclusions : These results show that a more intense antigen-independent inflammation develops with recruitment of both neutrophils and T lymphocytes of helper/inducer phenotype in the conjunctiva of patients with Behçet disease in response to surgical trauma. Increased expression of E-selectin and ICAM-1 in the conjunctiva of patients with Behçet disease may suggest a critical role for these adhesion molecules in the initial events of inflammation.

Revue / Journal Title

Ophthalmology   ISSN 0161-6420   CODEN OPHTDG 

Source / Source

1995, vol. 102, n^o11, pp. 1660-1668 (55 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, New York, NY, ETATS-UNIS  (1976) (Revue)

Mots-clés anglais / English Keywords

Conjunctiva ; Uvea ; Immunological investigation ; Pathology ; Immunohistochemistry ; Behçet syndrome ; Monoclonal antibody ; Uvea disease ; Eye disease ; Skin disease ; Stomatology ; Genital diseases ; Conjunctiva disease ;

Mots-clés français / French Keywords

Conjonctive ; Uvée ; Exploration immunologique ; Anatomopathologie ; Immunohistochimie ; Oculomucocutané Behçet syndrome ; Anticorps monoclonal ; Uvée pathologie ; Oeil pathologie ; Peau pathologie ; Stomatologie ; Appareil génital pathologie ; Conjonctive pathologie ;

Mots-clés espagnols / Spanish Keywords

Conjuntiva ; Uvea ; Análisis inmunológico ; Anatomía patológica ; Inmunohistoquímica ; Behçet síndrome ; Anticuerpo monoclonal ; Uvea patología ; Ojo patología ; Piel patología ; Estomatología ; Aparato genital patología ; Conjuntiva patología ;

Localisation / Location

INIST-CNRS, Cote INIST : 18914, 35400005892493.0160