Titre du document / Document title

A substance isolated from Cornus officinalis enhances the motility of human sperm

Auteur(s) / Author(s)

JENG H. ⁽¹⁾ ; CHAO MEI WU ⁽²⁾ ; SU S.-J. ⁽³⁾ ; CHANG W.-C. ^{(4 5)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Anatomy, Taipei Medical College, Taipei, TAIWAN, PROVINCE DE CHINE
⁽²⁾ Department of Pharmacy, Taipei Medical College, Taipei, TAIWAN, PROVINCE DE CHINE
⁽³⁾ Department of Pathology, Taipei Medical College, Taipei, TAIWAN, PROVINCE DE CHINE
⁽⁴⁾ Institute of Biochemical Sciences, National Taiwan University, Taipei, TAIWAN, PROVINCE DE CHINE
⁽⁵⁾ Institute of Biological Chemistry, Academia Sinica, Taipei, TAIWAN, PROVINCE DE CHINE

Résumé / Abstract

The effects of a Chinese herb, Cornus officinalis, on the motility of human sperm was studied. An aqueous extract was prepared from the dried fruits of the herb and used in this study. The crude extract at a final concentration of 0.5 μg/μl in phosphate buffered saline (pH 7.4) increased sperm motility from 25.8 ± 7.7% to 42.8 ± 10.3% (i.e. 68% increase, n = 7), as determined by the computer-aided-sperm-analysis (CASA) method. The crude extract was fractionated by high-performance liquid chromatography (HPLC) into four fractions: C1, C2, C3 and C4. Their effects on sperm motility were further studied by CASA. Only the C4 fraction showed substantial stimulatory effects on sperm motility. At a concentration of 5 ng/μl, C4 increased the sperm motility from 15.7 ± 3.8% to 34.5 ± 6.4% (i.e. 120% increase, n = 6) by CASA and from 14.9 ± 4.3 to 28.5 ± 8.1 (i.e. 91% increase, n = 8) by transmembrane migration ratio (TMMR) method. This result suggests that C4 is the active component in Cornus officinalis that enhances sperm motility.

Revue / Journal Title

The American journal of Chinese medicine   ISSN 0192-415X   CODEN AJCMBA 

Source / Source

1997, vol. 25, n^o3-4, pp. 301-306 (6 ref.)

Langue / Language

Anglais

Editeur / Publisher

Institute for Advanced Research in Asian Science and Medicine, Garden City, NY, ETATS-UNIS  (1979) (Revue)

Mots-clés anglais / English Keywords

Medicinal plant ; Folk medicine ; Chinese medicine ; Taiwan ; Extract ; Plant origin ; Biological activity ; Mobility ; Spermatozoa ; In vitro ; Asia ;

Mots-clés français / French Keywords

Plante médicinale ; Médecine traditionnelle ; Médecine chinoise ; Taiwan ; Extrait ; Origine végétale ; Activité biologique ; Mobilité ; Spermatozoïde ; In vitro ; cornus officinalis ; Asie ;

Mots-clés espagnols / Spanish Keywords

Planta medicinal ; Medicina tradicional ; Medicina china ; Taiwan ; Extracto ; Origen vegetal ; Actividad biológica ; Movilidad ; Espermatozoide ; In vitro ; Asia ;

Localisation / Location

INIST-CNRS, Cote INIST : 16517, 35400006982160.0060