RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Presence of enniatin B and its hepatic metabolites in plasma and liver samples from broilers and eggs from laying hens

Auteur(s) / Author(s)

IVANOVA L. (1) ; FAESTE C. K. (1) ; DELEZIE E. (2) ; VAN PAMEL E. (3) ; DAESELEIRE E. (3) ; CALLEBAUT A. (4) ; UHLIG S. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Norwegian Veterinary Institute, P.O. Box 750 Sentrum, 0106 Oslo, NORVEGE
(2) Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090 Melle, BELGIQUE
(3) Institute for Agricultural and Fisheries Research (ILVO), Animal Sciences Unit, Scheldeweg 68, 9090 Melle, BELGIQUE
(4) Veterinary and Agrochemical Research Centre (CODA-CERVA), Unit Toxins and Natural Components, Leuvensesteenweg 17, 3080 Tervuren, BELGIQUE

Résumé / Abstract

Enniatins, a large group of cyclodepsipeptides, are widely distributed contaminants of different crops intended for human and animal consumption. Enniatin B is one of the principal analogues in species of the genus Fusarium, known to have ionophoric, antibiotic, and insecticidal activity. Regardless of considerable cytotoxic effects observed in vitro, enniatins have been characterised as compounds with low acute toxicity in vivo. The biotransformation of enniatin B has previously been elucidated in liver microsomes, and 12 different metabolites (M1 to M12) have been reported. In order to provide a better basis for understanding the potential toxic effects in humans and animals, different samples (eggs, livers, plasma) from two different feeding studies have been analysed for the presence of enniatin B and its hepatic metabolites. The earlier reported metabolite M11, and a novel metabolite (designated M13), were dominant in liver samples from enniatin B exposed broilers. The peak area corresponding to the sodiated molecular ion of M11 was approximately 2.5 times larger than that of parent enniatin B in liver samples collected after one week of exposure. The same metabolites were also present in serum samples. In egg samples, only metabolites M13 and M4 were detected. The comparison of mass spectrometric data of M13 and enniatin B suggested that M13 is a monohydroxylated metabolite. The hepatic biotransformation of enniatin B was also investigated in vitro in chicken microsomes demonstrating good correlation with the metabolite profiles in the chicken samples. The results of the present study demonstrated an extensive biotransformation of enniatin B in vivo confirming previously reported in vitro data.

Revue / Journal Title

World mycotoxin journal    ISSN  1875-0710 

Source / Source

2014, vol. 7, no2, pp. 167-175 [9 page(s) (article)] (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Wageningen Academic Publishers, Wageningen, PAYS-BAS  (2008) (Revue)

Mots-clés anglais / English Keywords

Poultry

;

Toxin

;

Vertebrata

;

Aves

;

Meat animals

;

Mycotoxin

;

In vivo

;

Liquid chromatography

;

Microsome

;

Metabolism

;

Hen

;

Egg laying

;

Chicken

;

Sample

;

Metabolite

;

Liver

;

Mots-clés français / French Keywords

Volaille

;

Toxine

;

Vertebrata

;

Aves

;

Animal à viande

;

Mycotoxine

;

In vivo

;

Chromatographie phase liquide

;

Microsome

;

Métabolisme

;

Poule

;

Ponte

;

Poulet

;

Echantillon

;

Métabolite

;

Foie

;

Mots-clés espagnols / Spanish Keywords

Ave de corral

;

Toxina

;

Vertebrata

;

Aves

;

Animales de carne

;

Micotoxina

;

In vivo

;

Cromatografía fase líquida

;

Microsoma

;

Metabolismo

;

Gallina

;

Puesta

;

Pollo

;

Muestra

;

Metabolito

;

Hígado

;

Mots-clés d'auteur / Author Keywords

enniatins

;

metabolism

;

liver microsomes

;

LC-MS

;

in vivo

;

Localisation / Location

INIST-CNRS, Cote INIST : 28127, 35400050042572.0070

Nº notice refdoc (ud4) : 28352622



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas