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Titre du document / Document title

Pyruvate alleviates toxic effects of ethanol on cells in culture

Auteur(s) / Author(s)

FUKAYA K. (1) ; MIYAZAKI M. (1) ; PU H. (1) ; KATAYAMA B. (1) ; INOUE Y. (1) ; OHASHI R. (1) ; NAKAMURA C. (2) ; NAMBA M. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama University Medical School, Okayama 700, JAPON
(2) Department of Legal Medicine, Okayama University Medical School, Okayama 700, JAPON

Résumé / Abstract

Cytotoxic effects of ethanol on cultured human hepatocytes and fibroblasts differed with the type of culture medium used. When cytotoxic effects of ethanol were compared between culture systems using either RPMI-1640 or Dulbecco's modified Eagle's medium (DMEM), the cytotoxicity was more prominent with the former medium than with the latter. A reduction of the cytotoxic effects appeared to be due to the pyruvate contained in DMEM. The protective effect of pyruvate against ethanol toxicity may be related to tricarboxylic acid (TCA) cycle activity because addition of malate to the medium also reduced the cytotoxic effects. Our results suggest that drug cytotoxicity testing in vitro must be done using various types of culture medium.

Revue / Journal Title

Archives of toxicology    ISSN  0340-5761   CODEN ARTODN 

Source / Source

1997, vol. 71, no10, pp. 651-654 (10 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1974) (Revue)

Mots-clés anglais / English Keywords

Ethanol

;

Toxicity

;

In vitro

;

Cell culture

;

Hepatocyte

;

Fibroblast

;

Culture medium

;

Pyruvate

;

Interference

;

Interaction

;

Drug

;

Cytotoxicity test

;

Investigation method

;

Mots-clés français / French Keywords

Ethanol

;

Toxicité

;

In vitro

;

Culture cellulaire

;

Hépatocyte

;

Fibroblaste

;

Milieu culture

;

Pyruvate

;

Interférence

;

Interaction

;

Médicament

;

Test cytotoxicité

;

Méthode étude

;

Mots-clés espagnols / Spanish Keywords

Etanol

;

Toxicidad

;

In vitro

;

Cultivo celular

;

Hepatocito

;

Fibroblasto

;

Medio cultivo

;

Piruvato

;

Interferencia

;

Interacción

;

Medicamento

;

Prueba citotoxicidad

;

Método estudio

;

Localisation / Location

INIST-CNRS, Cote INIST : 7527, 35400006902713.0080

Nº notice refdoc (ud4) : 2797197



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