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Titre du document / Document title

Immunomodulatory function and in vivo properties of Pediococcus pentosaceus OZF, a promising probiotic strain

Auteur(s) / Author(s)

Osmanagaoglu Ozlem (1) ; Kiran Fadime (1) ; Yagci Fuat C. (2) ; Gursel Ihsan (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Biology, Faculty of Science, Ankara University, 06100, Ankara, Turkey
(2) Department of Molecular Biology and Genetics, Therapeutic ODN Research Laboratory, Bilkent University, Ankara, Turkey

Résumé / Abstract

Some of the important properties of probiotics are the ability to survive during gastrointestinal transit and to modulate the immune functions. The objectives of the reported study were to assess in vivo gastrointestinal survival of orally administered Pediococcus pentosaceus OZF using an animal model BALB/c mice, and to examine its effects on the immune response. Following oral administration to mice, the ability of Pediococcus pentosaceus OZF to pass and survive through the mouse gastrointestinal system was investigated by analyzing the recovery of the strain in fecal samples. Microbiological and polymerase chain reaction (PCR) methods proved that the strain OZF could overcome specific conditions in the gastrointestinal tract of mice and reach the intestine alive after ingestion. To observe the effect of oral administration on immune response, IL-6, IL-12 and IFN-γ were measured by ELISA, and the strain OZF was found to cause increases in IL-6 synthesis in regularly fed mice. However, stimulation was carried out with various concentrations of bacterial ssDNA and heat killed cells of Pediococcus pentosaceus OZF. The heat killed cells of the strain OZF were shown to produce IFN-γ independently from IL-12. On the other hand, a significant difference between control and experimental group was noticed when lipopolysaccharide, a TLR4 (toll like receptor) ligand, was used. Overall, Pediococcus pentosaceus OZF may be a valuable probiotic strain for therapeutic uses. Nevertheless, further studies on the mechanisms of immunomodulatory effect will allow for better clarification of the immune functions of this strain.

Revue / Journal Title

Annals of microbiology    ISSN  1869-2044 

Source / Source

2013, vol. 63, no4, pp. 1311-1318 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (19?) (Revue)

Mots-clés d'auteur / Author Keywords

Probiotic

;

Survival

;

Immunomodulation

;

In vivo

;

Localisation / Location

35400061957057.0012

Springer-Verlag Berlin Heidelberg and the University of Milan, 2013
Nº notice refdoc (ud4) : 27858060



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