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Titre du document / Document title

Silver and hydrogen peroxide as potential drinking water disinfectants : Their bactericidal effects and possible modes of action

Auteur(s) / Author(s)

PEDAHZUR R. (1) ; SHUVAL H. I. (1) ; ULITZUR S. (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Environmental Sciences, The Hebrew University of Jerusalem, Jerusalem, ISRAEL
(2) Department of Food Engineering and Biotechnology, Technion, Haifa 32000, ISRAEL

Résumé / Abstract

Silver and hydrogen peroxide (HP) acted synergistically on the viability of E. coli K-12. In certain concentration ranges the synergistic effect amounted to about 3 logs. Toxicity process kinetics were determined by following the decrease in luminescence of a highly luminescent recombinant E. coli harbouring a plasmid carrying the whole lux system of Vibria fischeri. As in the viability studies, silver and HP also showed a synergistic effect on in vivo luminescence, which amounted to a 2 log increase in toxicity. A similar phenomenon was found for silver and certain metal ions, including CUZ+. NiZ+, Zn2+and Cd2+, where toxicity increased by a factor of 10 in the presence of sub-inhibitory concentrations of silver. To monitor the expression of different stress response systems in treated cells, we have used E. coli carrying fusions of the lux system to promoters of different stress-controlling genes. Of the fusions tested, HP substantially increased the activity of recA, katG, nucF, grpE, ion, and dnaK. Silver exerted a mild effect acting only on grpE and ion promoters. When in combination, a synergistic induction of the dnaK fusion and a slightly additive effect on grpE fusion were recorded. It appears that the comhined toxic effect of silver and HP may be related with damage to cellular proteins. Nevertheless, the involvement of other cellular moieties can not be ruled out. The possibility that the synergistic effect is related to chemical interactions between silver and HP and the consequent increase in their toxicity is discussed.

Revue / Journal Title

Water science and technology    ISSN  0273-1223   CODEN WSTED4 

Source / Source

Congrès
Health-related water microbiology 1996 :   ( Mallorca, 6-10 October 1996 )
IAWQ international symposium on health-related water microbiology No8, Mallorca , ESPAGNE (06/10/1996)
1997  , vol. 35, no 11-12, pp. 87-93[Note(s) : XI, 481 p., ] (17 ref.) ISBN 0-08-043300-6 ;  Illustration : Illustration ;

Langue / Language

Anglais

Editeur / Publisher

International Water Association, London, ROYAUME-UNI  (1981) (Revue)
Pergamon Press, Oxford, ROYAUME-UNI  ETATS-UNIS  (1997) (Monographie)

Mots-clés anglais / English Keywords

Drinking water treatment

;

Disinfection

;

Silver

;

Hydrogen Peroxides

;

Bactericidal effect

;

Escherichia coli

;

Synergism

;

Experimental study

;

Enterobacteriaceae

;

Bacteria

;

Mots-clés français / French Keywords

Traitement eau potable

;

Désinfection

;

Argent

;

Hydrogène Peroxyde

;

Bactéricidie

;

Escherichia coli

;

Synergie

;

Etude expérimentale

;

Enterobacteriaceae

;

Bactérie

;

Mots-clés espagnols / Spanish Keywords

Tratamiento agua potable

;

Desinfección

;

Plata

;

Hidrógeno Peróxido

;

Bactericidia

;

Escherichia coli

;

Sinergia

;

Estudio experimental

;

Enterobacteriaceae

;

Bacteria

;

Localisation / Location

INIST-CNRS, Cote INIST : 16441, 35400006808373.0160

Nº notice refdoc (ud4) : 2733378



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