Titre du document / Document title

Determination of parishin, parishins B and C in traditional Chinese medicinal preparations by high performance liquid chromatography

Auteur(s) / Author(s)

KU Y.-R. ⁽¹⁾ ; LIN Y.-T. ⁽¹⁾ ; WEN K.-C. ⁽¹⁾ ; LIN J.-H. ⁽¹⁾ ; LIAO C.-H. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ National Laboratories of Foods and Drugs, Department of Health, Executive Yuan, 161-2 Ruen-Yang Street, Nankang, Taipei, TAIWAN, PROVINCE DE CHINE

Résumé / Abstract

High performance liquid chromatographic methods were established for the determination of parishin, parishins B and C in three kinds of the traditional Chinese medicinal prescriptions, including Gastrodiae Rhizoma : Shuen-Feng-Iun-Chi-San, Ban-Shia-Bai-Ju-Tian-Ma-Tang and Tsan-Siang-Tian-Ma-Tang. The samples were analyzed with an Inertsil ODS-2 reversed phase column by gradient elution with varied 0.1% (v/v) phosphoric acid-acetonitrile as mobile phases and detected at UV 222 nm. Ethoxybenzamide was used as an internal standard. Regression equations were derived showing linear relationships (correlation coefficients : 0.9976-0.9999) between the peak area ratios of each marker (parishin, parishins B and C) to ethoxybenzamide and concentration. The recoveries of parishin, parishins B and C from the Chinese medicinal preparations were 102.0-104.1%, 102.9-105.0% and 103.2-104.0%, respectively. The relative standard deviations of three marker constituents ranged between 0.90-2.47% (intraday) and 1.05-4.41% (interday). The contents of parishin, parishins B and C measured 1.58-2.85 mg/g, 1.58-3.25 mg/g and 1.45-1.72 mg/g, respectively.

Revue / Journal Title

Journal of liquid chromatography & related technologies   ISSN 1082-6076   CODEN JLCTFC 

Source / Source

1996, vol. 19, n^o20, pp. 3265-3277 (5 ref.)

Langue / Language

Anglais

Editeur / Publisher

Taylor & Francis, Colchester, ROYAUME-UNI  (1996) (Revue)

Mots-clés anglais / English Keywords

Chemical analysis ; Quantitative analysis ; Decoction ; Plant origin ; Rhizome ; Orchidaceae ; Medicinal plant ; Folk medicine ; China ; Pharmacognosy ; Carboxylic acid ; Ester ; Glycoside ; Aldose ; Active ingredient ; HPLC chromatography ; Reversed phase chromatography ; Monocotyledones ; Angiospermae ; Spermatophyta ; Asia ;

Mots-clés français / French Keywords

Analyse chimique ; Analyse quantitative ; Tisane ; Origine végétale ; Rhizome ; Orchidaceae ; Plante médicinale ; Médecine traditionnelle ; Chine ; Pharmacognosie ; Acide carboxylique ; Ester ; Glycoside ; Aldose ; Principe actif ; Chromatographie HPLC ; Chromatographie phase inverse ; Parishine ; Gastrodia elata ; Propane-1,2,3-tricarboxylique acide dérivé ; Glucopyranoside dérivé ; Monocotyledones ; Angiospermae ; Spermatophyta ; Asie ;

Mots-clés espagnols / Spanish Keywords

Análisis químico ; Análisis cuantitativo ; Tisana ; Origen vegetal ; Rizoma ; Orchidaceae ; Planta medicinal ; Medicina tradicional ; China ; Farmacognosia ; Acido carboxílico ; Ester ; Glicósido ; Aldosa ; Principio activo ; Cromatografía HPLC ; Cromatografía fase inversa ; Monocotyledones ; Angiospermae ; Spermatophyta ; Asia ;

Localisation / Location

INIST-CNRS, Cote INIST : 18068, 35400006096532.0030