Titre du document / Document title
Simultaneous separation of diazepam and its chiral and achiral metabolites by HPLC on a Chiralcel OD-R column
Auteur(s) / Author(s)
LIU J.
(1) ;
STEWART J. T.
(1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Medicinal Chemistry, College of Pharmacy, The University of Georgia, Athens, GA 30602-2352, ETATS-UNIS
Résumé / Abstract
A reversed-phase HPLC method was developed for the simultaneous separation of diazepam and its achiral (nordiazepam) and chiral (oxazepam and temazepam) metabolites. Chromatographic resolution of the analytes was performed under isocratic conditions using a mobile phase of 0.3 M sodium perchlorate-acetonitrile (57:43 v/v) at a flow rate of 0.5 ml/min on a reversed-phase cellulose-based chiral stationary phase (Chiralcel OD-R column) with UV detection set at 210 nm. Disc solid-phase extraction was carried out for serum sample clean-up prior to HPLC separation. Recoveries were determined for diazepam, nordiazepam, and oxazepam and temazepam enantiomers from human serum at 300 ng/ml levels. The limits of detection were 25 ng/ml each for diazepam and nordiazepam, and about 25 ng/ml for each enantiomer of oxazepam and temazepam (S/N = 2). The method can be applicable to the in vivo analysis of human serum samples containing diazepam and its chiral and achiral metabolites.
Revue / Journal Title
Analytical letters
ISSN 0003-2719
CODEN ANALBP
Source / Source
1997, vol. 30, n
o8, pp. 1555-1566 (13 ref.)
Langue / Language
Anglais
Editeur / Publisher
Taylor & Francis, Philadelphia, PA, ETATS-UNIS
(1967)
(Revue)
Mots-clés anglais / English Keywords
Quantitative analysis ;
Simultaneous measurement ;
Diazepam ;
Metabolite ;
Nordazepam ;
Oxazepam ;
Temazepam ;
Benzodiazepine derivatives ;
Psychotropic ;
Tranquillizer ;
Chirality ;
Enantiomer ;
In vivo ;
Biological fluid ;
Serum ;
Human ;
HPLC chromatography ;
Ultraviolet detector ;
Mots-clés français / French Keywords
Analyse quantitative ;
Mesure simultanée ;
Diazépam ;
Métabolite ;
Nordazépam ;
Oxazépam ;
Témazépam ;
Benzodiazépine dérivé ;
Psychotrope ;
Tranquillisant ;
Chiralité ;
Enantiomère ;
In vivo ;
Liquide biologique ;
Sérum ;
Homme ;
Chromatographie HPLC ;
Détecteur UV ;
Mots-clés espagnols / Spanish Keywords
Análisis cuantitativo ;
Medición simultánea ;
Diazepam ;
Metabolito ;
Nordazepam ;
Oxazepam ;
Temazepam ;
Benzodiazepina derivado ;
Psicotropo ;
Tranquilizante ;
Quiralidad ;
Enantiómero ;
In vivo ;
Líquido biológico ;
Suero ;
Hombre ;
Cromatografía HPLC ;
Detector UV ;
Localisation / Location
INIST-CNRS, Cote INIST : 13703, 35400006742176.0100
Nº notice refdoc (ud4) : 2719062
