Titre du document / Document title
Phenotypic variations and switches in HIV isolated from the blood and the gastrointestinal tissues of patients with HIV-1 infection
Auteur(s) / Author(s)
HIV/GI Research Study Group
AL-MULLA W. (1 2) ;
CHURCH D. (2) ;
GILL M. J. (2) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Faculty of Medicine, Kuwait University, KOWEIT
(2) Departments of Medicine and Microbiology and Infectious Diseases, Faculty of Medicine, University of Calgary, Alberta, CANADA
Résumé / Abstract
The objective of this study was to determine the initial and subsequent phenotypes of HIV-1 isolated from the blood, duodenal, and colonic biopsies of 51 HIV-1 positive patients followed prospectively over 2 years. Blood and tissues were cocultured with stimulated peripheral blood monocytes, and HIV was analyzed for phenotypic expression of syncytia-induction (SI). A total of 45/51 patients had HIV-1 isolated from the blood and 35/51 had HIV isolated from gastrointestinal tract. In 12/45 patients SI-HIV-1 was isolated from the blood. In 6/12 patients the blood phenotype reverted to the NSI phenotype. SI phenotypes were also isolated from the colon and duodenum of 8/35 patients and reversion from SI to NSI virus phenotype was again observed in gut tissue of 3/8 patients. These results show that gastrointestinal tissues can harbor SI HIV phenotype. Discordant phenotypes can be found in tissue and blood of late-stage patients. Reversion of phenotypic SI expression to NSI may occur in patients receiving monotherapy as antiretroviral treatment. These results suggest that gastrointestinal tissues may act as a separate and distinct site involved in HIV replication and its associated pathogenesis.
Revue / Journal Title
Journal of medical virology
ISSN
0146-6615
CODEN JMVIDB
Source / Source
1997, vol. 52, n
o1, pp. 31-34 (14 ref.)
Langue / Language
Anglais
Editeur / Publisher
Wiley, Hoboken, NJ, ETATS-UNIS
(1977)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 17422, 35400006528278.0060
Nº notice refdoc (ud4) : 2645183
