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Titre du document / Document title

β-lactam antibiotics inhibit chloroplast division in a moss (Physcomitrella patens) but not in tomato (Lycopersicon esculentum)

Auteur(s) / Author(s)

KASTEN B. (1) ; RESKI R. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Institut für Allgemeine Botanik, Ohnhorststr. 18, 22609 Hamburg, ALLEMAGNE

Résumé / Abstract

Photoautotrophic suspension cultures of moss protonema of Physcomitrella patens (Hedw.) B.S.G. and of tomato (Lycopersicon esculentum L.), respectively, were treated with three different β-lactam antibiotics. Ampicillin, cefotaxime and penicillin in the range from 10 μmol/L to 1 mmol/L. had no visible effects on tomato cells but led to moss cells with giant chloroplasts. According to electron micrographs these cells are morphologically normal but contain 2-3 macrochloroplasts with well developed inner membranes. As only chloroplasts in dividing tip cells were affected, we conclude that β-lactam antibiotics specifically inhibit the plastid division process in the moss. Major proteins were not affected by antibiotic treatments as judged by SDS-PAGE and subsequent silver stain. The inhibitory effect on chloroplast division could not be compensated for by exogenous cytokinin (5 X 10-6 mol/L i6Ade). However, upon removal of the antibiotics, macrochloroplasts started to divide. As division of bacteria and of cyanelles is sensitive to β-lactam antibiotics, we suggest that mosses conserved an ancient component of the chloroplast division mechanism that was subsequently modified or substituted during plastid evolution in land plants.

Revue / Journal Title

Journal of plant physiology    ISSN  0176-1617   CODEN JPPHEY 

Source / Source

1997, vol. 150, no1-2, pp. 137-140 (20 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Munich, ALLEMAGNE  (1984) (Revue)

Mots-clés anglais / English Keywords

Cell culture

;

Pretreatment

;

Lactam

;

Antibiotic

;

Chloroplast

;

Division

;

Inhibition

;

Ultrastructure

;

Lycopersicon esculentum

;

Solanaceae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Cell organelle

;

Musci

;

Bryophyta

;

Mots-clés français / French Keywords

Culture cellulaire

;

Prétraitement

;

Lactame

;

Antibiotique

;

Chloroplaste

;

Division

;

Inhibition

;

Ultrastructure

;

Lycopersicon esculentum

;

Physcomitrella patens

;

Solanaceae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Organite cellulaire

;

Musci

;

Bryophyta

;

Mots-clés espagnols / Spanish Keywords

Cultivo celular

;

Pretratamiento

;

Lactamo

;

Antibiótico

;

Cloroplasto

;

División

;

Inhibición

;

Ultraestructura

;

Lycopersicon esculentum

;

Solanaceae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Organulo celular

;

Musci

;

Bryophyta

;

Localisation / Location

INIST-CNRS, Cote INIST : 922, 35400006488689.0220

Nº notice refdoc (ud4) : 2640663



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