Titre du document / Document title
β-lactam antibiotics inhibit chloroplast division in a moss (Physcomitrella patens) but not in tomato (Lycopersicon esculentum)
Auteur(s) / Author(s)
KASTEN B. (1) ;
RESKI R. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Institut für Allgemeine Botanik, Ohnhorststr. 18, 22609 Hamburg, ALLEMAGNE
Résumé / Abstract
Photoautotrophic suspension cultures of moss protonema of Physcomitrella patens (Hedw.) B.S.G. and of tomato (Lycopersicon esculentum L.), respectively, were treated with three different β-lactam antibiotics. Ampicillin, cefotaxime and penicillin in the range from 10 μmol/L to 1 mmol/L. had no visible effects on tomato cells but led to moss cells with giant chloroplasts. According to electron micrographs these cells are morphologically normal but contain 2-3 macrochloroplasts with well developed inner membranes. As only chloroplasts in dividing tip cells were affected, we conclude that β-lactam antibiotics specifically inhibit the plastid division process in the moss. Major proteins were not affected by antibiotic treatments as judged by SDS-PAGE and subsequent silver stain. The inhibitory effect on chloroplast division could not be compensated for by exogenous cytokinin (5 X 10
-6 mol/L i
6Ade). However, upon removal of the antibiotics, macrochloroplasts started to divide. As division of bacteria and of cyanelles is sensitive to β-lactam antibiotics, we suggest that mosses conserved an ancient component of the chloroplast division mechanism that was subsequently modified or substituted during plastid evolution in land plants.
Revue / Journal Title
Journal of plant physiology
ISSN
0176-1617
CODEN JPPHEY
Source / Source
1997, vol. 150, n
o1-2, pp. 137-140 (20 ref.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, Munich, ALLEMAGNE
(1984)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 922, 35400006488689.0220
Nº notice refdoc (ud4) : 2640663
