Titre du document / Document title

Accumulation of diterpenoids in cell and root-organ cultures of Jatropha species

Auteur(s) / Author(s)

PLETSCH M. ⁽¹⁾ ; CHARLWOOD B. V. ^{(1 2)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Laboratório de Biotecnologia de Produtos Naturais, Departamento de Quimica, Universidade Federal de Alagoas, Campus Universitário, 57072-970 Maceió-AL, BRESIL
⁽²⁾ Division of Life Sciences, King's College London, Campden Hill Road, Kensington, London W8 7AH, ROYAUME-UNI

Résumé / Abstract

In a number of species of the genus Jatropha, used in folk medicine in tropical areas, the active principle is the macrocyclic diterpenoid jatrophone, which co-occurs with the inactive diterpenoids jatropholone A and B. Callus and suspension cultures of Jatropha cathartica, J. cinerea, J. curcas, J. dioica, J. elliptica, J. multifida, J. podagrica and J. mollissima were established, but most of the cell lines tested accumulated no, or only small quantities of jatrophone. The maximum jatrophone accumulation (3 μg. g^-l dry weight) was obtained with callus and suspension cultures derived from J. curcas and J. elliptica. Root-organ cultures of J. elliptica formed following incubation of suspension cells in the presence of 3-indolebutyric acid (5 mg. L^-l). The maximum accumulation of jatrophone and the jatropholones in newly initiated roots grown in vitro was 329 and 458 μg. g^-l dry weight, respectively. The effects on diterpenoid accumulation by root-organs of alteration of environmental parameters, and of the addition of chitosan, Benomyl and Al³⁺ to the growth medium were investigated. The yield of jatrophone was significantly increased in the presence of 0.07-0.7 mmol/L Al³⁺, and the product profile was altered concomitantly.

Revue / Journal Title

Journal of plant physiology   ISSN 0176-1617   CODEN JPPHEY 

Source / Source

1997, vol. 150, n^o1-2, pp. 37-45 (28 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Jena, ALLEMAGNE  (1984) (Revue)

Mots-clés anglais / English Keywords

Diterpene ; Biological accumulation ; Cell culture ; Callus ; Aluminium III ; Regulation(control) ; Jatropha ; Euphorbiaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Secondary metabolism ; Medicinal plant ;

Mots-clés français / French Keywords

Jatrophone ; Diterpène ; Accumulation biologique ; Culture cellulaire ; Cal ; Aluminium III ; Régulation ; Jatropha ; Jatropholone ; Euphorbiaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Métabolisme secondaire ; Plante médicinale ;

Mots-clés espagnols / Spanish Keywords

Diterpeno ; Acumulación biológica ; Cultivo celular ; Callo ; Aluminio III ; Regulación ; Jatropha ; Euphorbiaceae ; Dicotyledones ; Angiospermae ; Spermatophyta ; Metabolismo secundario ; Planta medicinal ;

Localisation / Location

INIST-CNRS, Cote INIST : 922, 35400006488689.0070