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Titre du document / Document title

Chitin degradation proteins produced by the marine bacterium Vibrio harveyi growing on different forms of chitin

Auteur(s) / Author(s)

SVITIL A. L. (1) ; NI CHADHAIN S. M. (1) ; MOORE J. A. (1) ; KIRCHMAN D. L. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) College of Marine Studies, University of Delaware, Lewes, Delaware 19958, ETATS-UNIS

Résumé / Abstract

Relatively little is known about the number, diversity, and function of chitinases produced by bacteria, even though chitin is one of the most abundant polymers in nature. Because of the importance of chitin, especially in marine environments, we examined chitin-degrading proteins in the marine bacterium Vibrio harveyi. This bacterium had a higher growth rate and more chitinase activity when grown on β-chitin (isolated from squid pen) than on α-chitin (isolated from snow crab), probably because of the more open structure of β-chitin. When exposed to different types of chitin, V. harveyi excreted several chitin-degrading proteins into the culture media. Some chitinases were present with all of the tested chitins, while others were unique to a particular chitin. We cloned and identified six separate chitinase genes from V. harveyi. These chitinases appear to be unique based on DNA restriction patterns, immunological data, and enzyme activity. This marine bacterium and probably others appear to synthesize separate chitinases for efficient utilization of different forms of chitin and chitin by-products.

Revue / Journal Title

Applied and environmental microbiology    ISSN  0099-2240   CODEN AEMIDF 

Source / Source

1997, vol. 63, no2, pp. 408-413 (24 ref.)

Langue / Language

Anglais

Editeur / Publisher

American Society for Microbiology, Washington, DC, ETATS-UNIS  (1976) (Revue)

Mots-clés anglais / English Keywords

Enzymatic activity

;

Chitinase

;

Vibrio harveyi

;

Degradation product

;

Chitin

;

Identification

;

Gene

;

Substrate specificity

;

O-Glycosidases

;

Glycosidases

;

Hydrolases

;

Enzyme

;

Vibrionaceae

;

Bacteria

;

Mots-clés français / French Keywords

Activité enzymatique

;

Chitinase

;

Vibrio harveyi

;

Produit dégradation

;

Chitine

;

Identification

;

Gène

;

Spécificité substrat

;

α-Chitine

;

β-Chitine

;

O-Glycosidases

;

Glycosidases

;

Hydrolases

;

Enzyme

;

Vibrionaceae

;

Bactérie

;

Mots-clés espagnols / Spanish Keywords

Actividad enzimática

;

Chitinase

;

Vibrio harveyi

;

Producto degradación

;

Quitina

;

Identificación

;

Gen

;

Especificidad sustrato

;

O-Glycosidases

;

Glycosidases

;

Hydrolases

;

Enzima

;

Vibrionaceae

;

Bacteria

;

Localisation / Location

INIST-CNRS, Cote INIST : 7195, 35400006292222.0080

Nº notice refdoc (ud4) : 2562374



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