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Titre du document / Document title

Low diversity of the gut microbiota in infants with atopic eczema

Auteur(s) / Author(s)

ABRAHAMSSON Thomas R. (1) ; JAKOBSSON Hedvig E. (2 3) ; ANDERSSON Anders F. (4) ; BJÖRKSTEN Bengt (5) ; ENGSTRAND Lars (2 3) ; JENMALM Maria C. (1 6) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Clinical and Experimental Medicine, Division of Pediatrics, Linkoping University, SUEDE
(2) Department of Preparedness, Swedish Institute for Communicable Disease Control, Solna, SUEDE
(3) Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, SUEDE
(4) Science for Life Laboratory, School of Biotechnology, KTH Royal Institute of Technology, Stockholm, SUEDE
(5) Institute of Environmental Medicine, Karolinska Institutet, Stockholm, and the School of Health and Medical Sciences, Örebro University, SUEDE
(6) Department of Clinical and Experimental Medicine, Unit of Autoimmunity and Immune Regulation, Division of Clinical Immunology, Linkoping University, SUEDE

Résumé / Abstract

Background: It is debated whether a low total diversity of the gut microbiota in early childhood is more important than an altered prevalence of particular bacterial species for the increasing incidence of allergic disease. The advent of powerful, cultivation-free molecular methods makes it possible to characterize the total microbiome down to the genus level in large cohorts. Objective: We sought to assess microbial diversity and characterize the dominant bacteria in stool during the first year of life in relation to atopic eczema development. Methods: Microbial diversity and composition were analyzed with barcoded 16S rDNA 454-pyrosequencing in stool samples at 1 week, 1 month, and 12 months of age in 20 infants with IgE-associated eczema and 20 infants without any allergic manifestation until 2 years of age (ClinicalTrials.gov ID NCT01285830). Results: Infants with IgE-associated eczema had a lower diversity of the total microbiota at 1 month (P = .004) and a lower diversity of the bacterial phylum Bacteroidetes and the genus Bacteroides at 1 month (P = .02 and P = .01) and the phylum Proteobacteria at 12 months of age (P = .02). The microbiota was less uniform at 1 month than at 12 months of age, with a high interindividual variability. At 12 months, when the microbiota had stabilized, Proteobacteria, comprising gram-negative organisms, were more abundant in infants without allergic manifestation (Empirical Analysis of Digital Gene Expression in R [edgeR] test: P = .008, q = 0.02). Conclusion: Low intestinal microbial diversity during the first month of life was associated with subsequent atopic eczema.

Revue / Journal Title

Journal of allergy and clinical immunology    ISSN  0091-6749   CODEN JACIBY 

Source / Source

2012, vol. 129, no2, pp. 434-440 [7 page(s) (article)] (35 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, New York, NY, ETATS-UNIS  (1971) (Revue)

Mots-clés anglais / English Keywords

Skin disease

;

Digestive system

;

Bacteria

;

Bacteroidaceae

;

Human

;

Immunopathology

;

Immunology

;

Microbiology

;

Hypothesis

;

Hygiene

;

Species

;

Bacteroides

;

Infant

;

Gut

;

Allergy

;

Eczema

;

Atopy

;

Mots-clés français / French Keywords

Pathologie de la peau

;

Appareil digestif

;

Bactérie

;

Bacteroidaceae

;

Homme

;

Immunopathologie

;

Immunologie

;

Microbiologie

;

Hypothèse

;

Hygiène

;

Espèce

;

Bacteroides

;

Nourrisson

;

Intestin

;

Allergie

;

Eczéma

;

Atopie

;

Mots-clés espagnols / Spanish Keywords

Piel patología

;

Aparato digestivo

;

Bacteria

;

Bacteroidaceae

;

Hombre

;

Inmunopatología

;

Inmunología

;

Microbiología

;

Hipótesis

;

Higiene

;

Especie

;

Bacteroides

;

Lactante

;

Intestino

;

Alergia

;

Eczema

;

Atopía

;

Mots-clés d'auteur / Author Keywords

Allergic disease

;

Bacteroides species

;

diversity

;

eczema

;

hygiene hypothesis

;

infant

;

microbiota

;

molecular microbiology

;

pyrosequencing

;

Sutterella species

;

Localisation / Location

INIST-CNRS, Cote INIST : 2059, 35400050287102.0200

Nº notice refdoc (ud4) : 25595640



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