RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Prospective comparison of commercially available rapid urease tests for the diagnosis of Helicobacter pylori

Auteur(s) / Author(s)

LAINE L. (1) ; LEWIN D. (1) ; NARITOKU W. (1) ; ESTRADA R. (1) ; COHEN H. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) University of Southern California School of Medicine, Los Angeles, California, ETATS-UNIS

Résumé / Abstract

Background : Rapid urease testing is the initial endoscopic test of choice for the diagnosis of Helicobacter pylori. Determination of the relative diagnostic yields and times to a positive test for the different rapid urease tests is important for endoscopists. We compared three commercially available tests using histologic examination and culture as a gold standard. Methods : Patients undergoing upper endoscopy had six biopsy specimens taken from the antrum and six from the body with a large-channel biopsy forceps. Each set of six specimens was divided as follows : one each for CLOtest, Hpfast, and Pyloritek rapid urease tests ; one for culture ; and two for histologic examination (H&E, Genta). All tests were read every 15 minutes for 1 hour ; the final reading for Pyloritek was at 1 hour. CLOtest and Hpfast were also read at 4 hours and 24 hours. Results : One hundred seventy-three sets of biopsy specimens from 87 patients were evaluated ; 98 (57%) of the 173 sets were positive for H. pylori by histologic examination and/or culture. The mean and median times to a positive test were significantly less for Pyloritek (0.5 ± 0.02 hour and 0.5 hour) than for CLOtest (2.0 ± 0.6 hour and 0.75 hour) or Hpfast (2.2 ± 0.6 hour and 0.5 hour). The sensitivities at the final readings were similar among the three tests (CLOtest : 93% ; Hpfast : 88% ; Pyloritek : 89%), but sensitivities at 1 hour were significantly better for Pyloritek (89%) than for CLOtest (71%) or Hpfast (66%). At 4 hours, sensitivities for CLOtest and Hpfast improved significantly and were not significantly different from those of Pyloritek. Specificities were 99% to 100% at all times for all three tests. Conclusion : The three rapid urease tests, CLOtest, Hpfast, and Pyloritek, provide comparable results, with sensitivities around 90% and specificities around 100%. The Pyloritek becomes positive more rapidly than the CLOtest or Hpfast. If a reading is desired within 1 hour, the Pyloritek provides a greater sensitivity than the CLOtest or Hpfast without any sacrifice in specificity.

Revue / Journal Title

Gastrointestinal endoscopy    ISSN  0016-5107   CODEN GAENBQ 

Source / Source

1996, vol. 44, no5, pp. 523-526 (5 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Maryland heights, MO, ETATS-UNIS  (1965) (Revue)

Mots-clés anglais / English Keywords

Gastritis

;

Campylobacter infection

;

Helicobacter pylori

;

Diagnosis

;

Microbiological investigation

;

Enzymatic activity

;

Urease

;

Laboratory investigations

;

Reagent strip

;

Sensitivity

;

Performance evaluation

;

Comparative study

;

Human

;

Bacteriosis

;

Infection

;

Spirillaceae

;

Spirillales

;

Bacteria

;

Hydrolases

;

Enzyme

;

Digestive diseases

;

Gastric disease

;

Biomedical engineering

;

Mots-clés français / French Keywords

Gastrite

;

Campylobactériose

;

Helicobacter pylori

;

Diagnostic

;

Exploration microbiologique

;

Activité enzymatique

;

Urease

;

Examen laboratoire

;

Bandelette réactive

;

Sensibilité

;

Evaluation performance

;

Etude comparative

;

Homme

;

Bactériose

;

Infection

;

Spirillaceae

;

Spirillales

;

Bactérie

;

Hydrolases

;

Enzyme

;

Appareil digestif pathologie

;

Estomac pathologie

;

Génie biomédical

;

Mots-clés espagnols / Spanish Keywords

Gastritis

;

Campilobacteriosis

;

Helicobacter pylori

;

Diagnóstico

;

Análisis microbiológico

;

Actividad enzimática

;

Urease

;

Examen laboratorio

;

Cinta reactiva

;

Sensibilidad

;

Evaluación prestación

;

Estudio comparativo

;

Hombre

;

Bacteriosis

;

Infección

;

Spirillaceae

;

Spirillales

;

Bacteria

;

Hydrolases

;

Enzima

;

Aparato digestivo patología

;

Estómago patología

;

Ingeniería biomédica

;

Localisation / Location

INIST-CNRS, Cote INIST : 16553, 35400006717541.0020

Nº notice refdoc (ud4) : 2494953



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas