Titre du document / Document title

Glycogen synthase regulation in hyperinsulinemic/obese progeny of rats fed a high carbohydrate formula in their infancy

Auteur(s) / Author(s)

SRINIVASAN M. ⁽¹⁾ ; VADLAMUDI S. ⁽¹⁾ ; PATEL M. S. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Biochemistry, School of Medicine and Biomedical Sciences, State University of New York at Buffalo, 3435 Main Street, Buffalo, NY 14214, ETATS-UNIS

Résumé / Abstract

OBJECTIVE : To evaluate the effects of chronic hyperinsulinemia/obesity on the proximal events leading to the activation of glycogen synthase. DESIGN : 100 d old second generation of chronically hyperinsulinemia/obese rats born to mothers which were artificially reared on a high carbohydrate (HC) milk formula in their infancy were used for this study and compared with mother-fed (MF) controls. MEASUREMENTS : Glycogen, glycogen synthase, protein phosphatase-1 (PP-1), mitogen-activated protein kinase (MAPK), insulin-stimulated protein kinase (ISPK) and protein kinase A (PKA) were measured in liver and muscle of both MF and HC rats. RESULTS : Glycogen content, glycogen synthase and PP-1 activities were significantly reduced in liver and muscle of HC rats compared to MF controls while trypsin released PP-1 activity was elevated. The activities of both MAPK and ISPK were also decreased in the HC rats. In contrast PKA activity was increased. CONCLUSIONS : Glycogen synthase activity in the basal state may be impaired in the hyperinsulinemic HC rats in two ways : (i) by a decrease in the activities of the kinases that presumably activate PP-1 and (ii) by increased activity of PKA which inactivates glycogen synthase directly by phosphorylation and indirectly by its effects on PP-1.

Revue / Journal Title

International journal of obesity   ISSN 0307-0565   CODEN IJOBDP 

Source / Source

1996, vol. 20, n^o11, pp. 981-989 (39 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing, Basingstoke, ROYAUME-UNI  (1977) (Revue)

Mots-clés anglais / English Keywords

Obesity ; Hyperinsulinemia ; Supplemented diet ; Carbohydrate ; Progeny ; Protein kinase ; Phosphoprotein phosphatase ; Experimental disease ; Animal ; Rat ; Nutritional status ; Transferases ; Enzyme ; Phosphoric monoester hydrolases ; Esterases ; Hydrolases ; Rodentia ; Mammalia ; Vertebrata ; Nutrition disorder ;

Mots-clés français / French Keywords

Obésité ; Hyperinsulinémie ; Régime alimentaire enrichi ; Glucide ; Descendance ; Protein kinase ; Phosphoprotein phosphatase ; Pathologie expérimentale ; Animal ; Rat ; Etat nutritionnel ; Transferases ; Enzyme ; Phosphoric monoester hydrolases ; Esterases ; Hydrolases ; Rodentia ; Mammalia ; Vertebrata ; Trouble nutrition ;

Mots-clés espagnols / Spanish Keywords

Obesidad ; Hiperinsulinemia ; Régimen alimenticio enriquecido ; Glúcido ; Descendencia ; Protein kinase ; Phosphoprotein phosphatase ; Patología experimental ; Animal ; Rata ; Estado nutricional ; Transferases ; Enzima ; Phosphoric monoester hydrolases ; Esterases ; Hydrolases ; Rodentia ; Mammalia ; Vertebrata ; Trastorno nutricíon ;

Localisation / Location

INIST-CNRS, Cote INIST : 18243, 35400006693114.0020