Titre du document / Document title

Prognostic value of morphometric features ant the callender classification in uveal melanomas

Auteur(s) / Author(s)

COLEMAN K. ⁽¹⁾ ; BAAK J. P. A. ⁽²⁾ ; VAN DIEST P. J. ⁽²⁾ ; MULLANEY J. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Research Pathology, The Royal Victoria Eye and Ear Hospital, Dublin, IRLANDE
⁽²⁾ Department of Pathology, The Free University Hospital, Amsterdam, PAYS-BAS

Résumé / Abstract

Purpose : Cytomorphometric analysis of uveal melanoma has focused on nucleolar characteristics, although nuclear measurements are easier and more reproducible. In this study, we examine a restricted set of nuclear and nucleolar cytomorphometric variables in the search for the most quantifiable and reproducible prognostic parameter among these and discover a means of assessing the malignancy of tumors within the different Callender subgroups. Methods : Uveal melanomas from 94 patients with a minimum follow-up of 5 years underwent nuclear and nucleolar morphometric analysis using a digitized interactive video overlay system. Statistical analysis included univariate and multivariate survival analysis on morphometric variables and classic prognostic parameters (such as Callender cell type, size, etc.). Results : Univariate analysis of cytomorphometric parameters showed the standard deviation of nuclear area (NASD) to be the most significant variable (P < 0.0001 ; Mantel-Cox, 15.2). This was followed by the difference between nuclear and nucleolar areas (P < 0.002 ; Mantel-Cox, 9.5), the standard deviation of nucleolar area (SDNA) (P < 0.01 ; Mantel-Cox, 6.4), and the standard deviation of the shortest nuclear axis (P < 0.02 ; Mantel-Cox, 6.4). There was a trend for significance with the mean of the ten largest nucleoli (P < 0.07). Multivariate analysis of clinical and cytomorphometric variables showed improvement of prognostic prediction with a combination of the NASD, largest tumor dimension, and glaucoma (P < 0.0001 ; Mantel-Cox, 26.3). Callender cell type was strongly correlated with the NASD (P < 0.0002), the mean axis ratio of the nuclear area (P < 0.0007), and the mean of the ten largest nucleoli (P < 0.0007). Conclusion : Cytomorphometric analysis of uveal melanoma should include nuclear area and may identify tumors of differing malignancy within the same Callender class.

Revue / Journal Title

Ophthalmology   ISSN 0161-6420   CODEN OPHTDG 

Source / Source

1996, vol. 103, n^o10, pp. 1634-1641 (23 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, New York, NY, ETATS-UNIS  (1976) (Revue)

Mots-clés anglais / English Keywords

Malignant melanoma ; Uvea ; Morphometry ; Cell nucleus ; Classification ; Prognosis ; Human ; Eye disease ; Malignant tumor ; Uvea disease ;

Mots-clés français / French Keywords

Mélanome malin ; Uvée ; Morphométrie ; Noyau cellulaire ; Classification ; Pronostic ; Homme ; Oeil pathologie ; Tumeur maligne ; Uvée pathologie ;

Mots-clés espagnols / Spanish Keywords

Melanoma maligno ; Uvea ; Morfometría ; Núcleo celular ; Clasificación ; Pronóstico ; Hombre ; Ojo patología ; Tumor maligno ; Uvea patología ;

Localisation / Location

INIST-CNRS, Cote INIST : 18914, 35400006686217.0200