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Titre du document / Document title

Purification and characterization of anthocyanin 3-aromatic acyltransferase from Perilla frutescens

Auteur(s) / Author(s)

FUJIWARA H. (1) ; TANAKA Y. (1) ; FUKUI Y. (1) ; ASHIKARI T. (1) ; YAMAGUCHI M. (2) ; KUSUMI T. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Institute for Fundamental Research, Suntory Ltd., 1-1-1, Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, JAPON
(2) Department of Food Science and Technology, Minami-Kyushu University, Takanabe, Miyazaki 884-0000, JAPON

Résumé / Abstract

Hydroxycinnamoyl-CoA:anthocyanin 3-O-glucoside-6-O-hydroxycinnamoyltransferase (3AT: EC 2.3.1.-) was identified and highly purified from red leaves of Perilla frutescens, which accumulate cyanidin 3-(6-O-p-coumaroyl-β- D-glucoside)-5-(6-O-malonyl-β-D-glucoside). 3AT was a 50 kDa monomer of protein with a pI of 5.3. It catalyzed the transfer of the p-coumaric and caffeic acids from their CoA esters to the 3-glucosyl moiety of delphinidin, cyanidin and pelargonidin 3-glucoside. Anthocyanidin 3.5-diglucosides were also good substrates. while cyanidin 3-rutinoside was a poor substrate. Malonyl-CoA was not used by the enzyme as an acyl-donor. Aromatic acylation of anthocyanin by 3AT caused a bathochromic shift. Lower K, values for anthocyanidin 3-glucoside than 3,5-diglucoside may indicate that acylation of cyanidin 3-glucoside precedes 5-O-glucosylation in vivo.

Revue / Journal Title

Plant science    ISSN  0168-9452   CODEN PLSCE4 

Source / Source

1998, vol. 137, no1, pp. 87-94 (19 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1985) (Revue)

Mots-clés anglais / English Keywords

Purification

;

Characterization

;

Acyltransferases

;

Anthocyanin

;

Plant leaf

;

Biosynthesis

;

Transferases

;

Enzyme

;

Enzymatic activity

;

Pigments

;

Labiatae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mots-clés français / French Keywords

Purification

;

Caractérisation

;

Acyltransferases

;

Anthocyane

;

Feuille végétal

;

Biosynthèse

;

Anthocyanin 3-aromatic acyltransferase

;

Perilla frutescens

;

Transferases

;

Enzyme

;

Activité enzymatique

;

Pigment

;

Labiatae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mots-clés espagnols / Spanish Keywords

Purificación

;

Caracterización

;

Acyltransferases

;

Antocianina

;

Hoja vegetal

;

Biosíntesis

;

Transferases

;

Enzima

;

Actividad enzimática

;

Pigmento

;

Labiatae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Localisation / Location

INIST-CNRS, Cote INIST : 15982, 35400007108484.0080

Nº notice refdoc (ud4) : 2414423



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