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Titre du document / Document title

TNF-α is a novel target of miR-19a

Auteur(s) / Author(s)

MEI LIU (1) ; ZAOZAO WANG (1) ; SHANGBIN YANG (1 2) ; WEI ZHANG (1) ; SHUN HE (1) ; CHENFEI HU (1) ; HONGXIA ZHU (1) ; LANPING QUAN (1) ; JINFENGBAI (1) ; NINGZHI XU (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Laboratory of Cell and Molecular Biology and State Key Laboratory of Molecular Oncology, Cancer Institute and Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, CHINE
(2) Department of Pathology, Ohio State University, 150 Hamilton Hall, 1645 Neil Ave., Columbus, OH 43210, ETATS-UNIS

Résumé / Abstract

Many studies have demonstrated the overexpression and amplification of the miR-17-92 cluster in malignant human cancers, including B-cell lymphomas and lung cancers. The purpose of this study was to investigate for the first time, the expression of the miR-17-92 cluster in esophageal squamous cell carcinoma (ESCC). The miR-17-92 cluster was found to be overexpressed in 21 out of 28 (75%) esophageal cancer samples. It was also found that overexpression of the miR-17-92 cluster could promote cellular growth in vivo and in vitro. Furthermore, inhibition of miR-19a by antisense oligonucleotides (ONs) induced apoptosis, while antisense ONs against miR-17-5p, miR-18a, miR-20a and miR-92-1 did not exhibit such an effect. In addition, it was found that antagomir-19a treatment could impair tumor growth in vivo. Using Human Apoptosis RT2 Profiler PCR Array 384HT, we found that tumor necrosis factor-α (TNF-α) was up-regulated 12-fold in cells transfected with miR-19a antisense ONs compared to the cells treated with the control scramble ONs. MiR-19a was predicted to target the 3' untranslated region of TNF-α mRNA, and this was confirmed by luciferase reporter assay. Taken together, we conclude that the miR-17-92 cluster is overexpressed in ESCC and that TNF-α is a novel target of miR-19a.

Revue / Journal Title

International journal of oncology    ISSN  1019-6439 

Source / Source

2011, vol. 38, no4, pp. 1013-1022 [10 page(s) (article)] (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

Editorial Academy of the International Journal of Oncology, Athens, GRECE  (1992) (Revue)

Mots-clés anglais / English Keywords

Cancer

;

Malignant tumor

;

Esophageal disease

;

Digestive diseases

;

Cytokine

;

Cancerology

;

Esophagus cancer

;

Targeting

;

Tumor necrosis factor α

;

Esophagus squamous cell carcinoma

;

Mots-clés français / French Keywords

Cancer

;

Tumeur maligne

;

Pathologie de l'oesophage

;

Pathologie de l'appareil digestif

;

Cytokine

;

Cancérologie

;

Cancer de l'oesophage

;

Ciblage

;

Facteur nécrose tumorale α

;

Carcinome épidermoïde de l'oesophage

;

Mots-clés espagnols / Spanish Keywords

Cáncer

;

Tumor maligno

;

Esófago patología

;

Aparato digestivo patología

;

Citoquina

;

Cancerología

;

Cáncer del esófago

;

Blancado

;

Factor necrosis tumoral α

;

Carcinoma epidermoide de esófago

;

Mots-clés d'auteur / Author Keywords

esophageal squamous cell carcinoma

;

miR-17-92 cluster

;

miR-19a

;

tumor necrosis factor-α

;

Localisation / Location

INIST-CNRS, Cote INIST : 26333, 35400019280487.0130

Nº notice refdoc (ud4) : 23927298



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