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Titre du document / Document title

Preparation, characterization, in vitro drug release and biological studies of curcumin loaded dextran sulphate―chitosan nanoparticles

Auteur(s) / Author(s)

ANITHA A. (1) ; DEEPAGAN V. G. (1) ; DIVYA RANI V. V. (1) ; MENON Deepthy (1) ; NAIR S. V. (1) ; JAYAKUMAR R. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Kochi 682041, INDE

Résumé / Abstract

Nanoformulation of curcumin, (a low molecular weight hydrophobic drug) was prepared by using dextran sulphate and chitosan. The developed nanoparticles were characterized by Dynamic Light Scattering measurements (DLS), Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FT-IR), X-ray Diffraction (XRD) and Differential Thermal Analysis (DTA). The prepared system showed an average size of 200―220 nm with a zeta potential value of -30 mV and showed ∼74% drug entrapment efficiency. In vitro drug release studies showed a controlled and pH dependent curcumin release over a period of one week. The cytocompatibility of bare nanoparticles was verified by MTT assay; cellular internalisation of curcumin loaded nanoparticles was confirmed by fluorescent imaging and quantified spectrophotometrically, anticancer activity of curcumin loaded nanoparticles was proved by MTT assay and reconfirmed by apoptosis assay (FACS). The results showed preferential killing of cancer cells compared to normal cells by the curcumin-loaded nanoparticles. Thus the developed curcumin loaded nanoformulation could be a promising candidate in cancer therapy. .

Revue / Journal Title

Carbohydrate polymers    ISSN  0144-8617   CODEN CAPOD8 

Source / Source

2011, vol. 84, no3, pp. 1158-1164 [7 page(s) (article)] (1/2 p.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1981) (Revue)

Mots-clés anglais / English Keywords

Oside polymer

;

Experimental study

;

Tumor cell

;

Biological activity

;

In vitro

;

Kinetics

;

Release

;

Internalization

;

Curcumin

;

Nanoencapsulation

;

Electrokinetic potential

;

Particle size distribution

;

Growth from solution

;

Mixing

;

Manufacturing

;

Antineoplastic agent

;

Drug carrier

;

Control release polymer

;

Chitosan

;

Interpolymer

;

Dextran derivatives

;

Nanoparticle

;

Mots-clés français / French Keywords

Oside polymère

;

Sulfate de dextrane

;

Etude expérimentale

;

Cellule tumorale

;

Activité biologique

;

In vitro

;

Cinétique

;

Libération

;

Internalisation

;

Curcumine

;

Nanoencapsulation

;

Potentiel électrocinétique

;

Distribution dimension particule

;

Méthode en solution

;

Mélangeage

;

Fabrication

;

Anticancéreux

;

Vecteur médicament

;

Polymère vecteur

;

Chitosane

;

Interpolymère

;

Dérivé du dextrane

;

Nanoparticule

;

Mots-clés espagnols / Spanish Keywords

Osido polímero

;

Estudio experimental

;

Célula tumoral

;

Actividad biológica

;

In vitro

;

Cinética

;

Liberación

;

Internalización

;

Curcumina

;

Nanoencapsulación

;

Potencial electrocinético

;

Distribución dimensión partícula

;

Método en solución

;

Mezclado

;

Fabricación

;

Anticanceroso

;

Vector medicamento

;

Polímero vector

;

Quitosano

;

Interpolímero

;

Dextrano derivado

;

Nanopartícula

;

Mots-clés d'auteur / Author Keywords

Chitosan

;

Dextran sulphate

;

Nanoparticles

;

Curcumin

;

Controlled delivery

;

Cytotoxicity

;

Cell uptake studies

;

Localisation / Location

INIST-CNRS, Cote INIST : 19272, 35400019461681.0410

Nº notice refdoc (ud4) : 23905778



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