Titre du document / Document title

Catechin-7-O-β-d-glucopyranoside scavenges free radicals and protects human B lymphoma BJAB cells on H₂O₂-mediated oxidative stress

Auteur(s) / Author(s)

Baek Jin-A ⁽¹⁾ ; Son Young-Ok ⁽²⁾ ; Fang Minghao ⁽¹⁾ ; Lee Young Jae ^{(3 4)} ; Cho Hyoung-Kwon ⁽³⁾ ; Whang Wan Kyunn ⁽⁴⁾ ; Lee Jeong-Chae ^{(1 5)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Institute of Oral Biosciences and BK21 Program, Chonbuk National University, 561-756, Jeonju, Jeonbuk, Korea
⁽²⁾ Graduate Center for Toxicology, College of Medicine, University of Kentucky, KY, Lexington, USA
⁽³⁾ Center for Health Care Technology Development, HanPoong Pharmaceutical Co., Ltd., 561-201, Jeonju, Jeonbuk, Korea
⁽⁴⁾ College of Pharmacy, Chung-Ang University, 156-756, Seoul, Korea
⁽⁵⁾ Research Center of Bioactive Materials, Institute of Oral Biosciences and BK21 Program, Chonbuk National University, 561-756, Jeonju, Jeonbuk, Korea

Résumé / Abstract

Catechin-7-O-β-d-glucopyranoside (CA-G) was previously isolated from red bean (the seed of Phaseolus calcaratus cv. Roxburgh). This study examined the ability of CA-G to scavenge reactive oxygen species generated by cell-free systems and to protect cells from oxidative stress caused by hydrogen peroxide (H₂O₂). The mechanism by which CA-G exerts its antioxidant and anti-apoptotic action on H₂O₂-exposed cells was also investigated. CA-G treatment prevented H₂O₂-mediated apoptosis and inhibited the formation of single stand breaks in DNA in H₂O₂-exposed BJAB cells. CA-G suppressed mitochondrial stress and caspase activation caused by H₂O₂. Mechanistic experiments revealed that the antioxidant mechanism of CA-G on H₂O₂-mediated oxidative damage was due to the direct scavenging of hydroxyl radicals and/or to the chelation of metal ions that were used to produce hydroxyl radicals from H₂O₂ via the Fenton reaction. Collectively, these findings suggest beneficial roles of CA-G or CA-G-rich red bean on the protection from oxidative damage.

Revue / Journal Title

Food science and biotechnology    ISSN  2092-6456 

Source / Source

2011, vol. 20, n^o1, pp. 151-158 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (2010) (Revue)

Mots-clés d'auteur / Author Keywords

apoptosis

;

catechin-7-O-β-d-glucopyranoside

;

DNA damage

;

hydrogen peroxide

;

oxidative stress

;

red bean

;

Localisation / Location

35400060712586.0021