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Titre du document / Document title

Catechin-7-O-β-d-glucopyranoside scavenges free radicals and protects human B lymphoma BJAB cells on H2O2-mediated oxidative stress

Auteur(s) / Author(s)

Baek Jin-A (1) ; Son Young-Ok (2) ; Fang Minghao (1) ; Lee Young Jae (3 4) ; Cho Hyoung-Kwon (3) ; Whang Wan Kyunn (4) ; Lee Jeong-Chae (1 5) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Institute of Oral Biosciences and BK21 Program, Chonbuk National University, 561-756, Jeonju, Jeonbuk, Korea
(2) Graduate Center for Toxicology, College of Medicine, University of Kentucky, KY, Lexington, USA
(3) Center for Health Care Technology Development, HanPoong Pharmaceutical Co., Ltd., 561-201, Jeonju, Jeonbuk, Korea
(4) College of Pharmacy, Chung-Ang University, 156-756, Seoul, Korea
(5) Research Center of Bioactive Materials, Institute of Oral Biosciences and BK21 Program, Chonbuk National University, 561-756, Jeonju, Jeonbuk, Korea

Résumé / Abstract

Catechin-7-O-β-d-glucopyranoside (CA-G) was previously isolated from red bean (the seed of Phaseolus calcaratus cv. Roxburgh). This study examined the ability of CA-G to scavenge reactive oxygen species generated by cell-free systems and to protect cells from oxidative stress caused by hydrogen peroxide (H2O2). The mechanism by which CA-G exerts its antioxidant and anti-apoptotic action on H2O2-exposed cells was also investigated. CA-G treatment prevented H2O2-mediated apoptosis and inhibited the formation of single stand breaks in DNA in H2O2-exposed BJAB cells. CA-G suppressed mitochondrial stress and caspase activation caused by H2O2. Mechanistic experiments revealed that the antioxidant mechanism of CA-G on H2O2-mediated oxidative damage was due to the direct scavenging of hydroxyl radicals and/or to the chelation of metal ions that were used to produce hydroxyl radicals from H2O2 via the Fenton reaction. Collectively, these findings suggest beneficial roles of CA-G or CA-G-rich red bean on the protection from oxidative damage.

Revue / Journal Title

Food science and biotechnology    ISSN  2092-6456 

Source / Source

2011, vol. 20, no1, pp. 151-158 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (2010) (Revue)

Mots-clés d'auteur / Author Keywords

apoptosis

;

catechin-7-O-β-d-glucopyranoside

;

DNA damage

;

hydrogen peroxide

;

oxidative stress

;

red bean

;

Localisation / Location

35400060712586.0021

The Korean Society of Food Science and Technology and Springer Netherlands, 2011
Nº notice refdoc (ud4) : 23809899



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