Titre du document / Document title

Tenascin-cytotactin (TN-C) variants in pseudophakic/aphakic bullous keratopathy corneas

Auteur(s) / Author(s)

MASERUKA H. ^{(1 2)} ; ATAULLAH S. M. ⁽¹⁾ ; ZARDI L. ⁽³⁾ ; TULLO A. B. ⁽¹⁾ ; RIDGWAY A. E. A. ⁽¹⁾ ; BONSHEK R. E. ^{(1 2)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Ophthalmology, Royal Eye Hospital, Manchester, ROYAUME-UNI
⁽²⁾ Department of Pathological Sciences, University of Manchester, Manchester, ROYAUME-UNI
⁽³⁾ Centro di Biotecnologie, Avenzate, Genoa, ITALIE

Résumé / Abstract

Purpose To examine pseudophakic/aphakic bullous keratopathy (PBK/ABK) human corneas for patterns of expression of tenascin-cytotactin (TN-C) variants known to mediate specific cellular functions, viz. anti-adhesion (high molecular mass (M_r)) and adhesion (low/ intermediate M_r). Methods PBK/ABK corneas were selected to encompass only those with bullae and without inflammation, scarring or neovascularisation. Serial sections from these and normal corneas were labelled with antibodies BC-4 (recognising all TN-C variants) and BC-2 (specific for the high M_r TN-C variant). Bound antibody was revealed with an avidin-biotin peroxidase technique. In a given pair of corneal sections, positivity with BC-4 but not BC-2 indicates localisation of low/ intermediate M_r TN-C variants and absence of the high M_r TN-C variant. BC-2 identifies the high M_rvariant. Results There was no immunostaining with either BC-2 or BC-4 in normal corneas except at the corneoscleral interface, where both BC-2 and BC-4 were immunolocalised. In PBK/ABK corneas, BC-2 staining was seen in 5 of 13 corneas and was restricted mainly to epithelial basement membrane (BM) overlying bullae. BC-2 did not label the stroma. BC-4 immunostaining was present in all PBK/ABK corneas and was localised in epithelial BM, both epithelial and stromal borders of bullae, pannus, endothelial BM and in oedematous stromal regions. Conclusions TN-C variants are differentially expressed in PBK/ABK corneas. The high M_r variant is restricted mainly to epithelial BM overlying bullae, while low/intermediate M_r variants occur in epithelial BM, both epithelial and stromal borders of bullae, and in pannus. Given the in vitro functions of TN-C, a role for promoting epithelial dehiscence and reattachment to the substratum in PBK/ABK corneas by high and low/intermediate M_r variants respectively is likely.

Revue / Journal Title

Eye   ISSN 0950-222X   CODEN EYEEEC 

Source / Source

1998, vol. 12 (4), pp. 729-734 (47 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing Group, Basingstoke, ROYAUME-UNI  (1987) (Revue)

Mots-clés anglais / English Keywords

Bullous keratopathy ; Aphaquia ; Protein ; Extracellular matrix ; Tenascin ; Gene expression ; Variant ; Cornea ; In vitro ; Human ; Eye disease ; Keratopathy ; Lens disease ;

Mots-clés français / French Keywords

Dystrophie cornéenne bulleuse ; Aphaquie ; Protéine ; Matrice extracellulaire ; Ténascine ; Expression génique ; Variant ; Cornée ; In vitro ; Homme ; Cytotactine ; Oeil pathologie ; Kératopathie ; Cristallin pathologie ;

Mots-clés espagnols / Spanish Keywords

Distrofia corneal bulosa ; Afaquia ; Proteína ; Matriz extracelular ; Tenascina ; Expresión genética ; Variante ; Córnea ; In vitro ; Hombre ; Ojo patología ; Queratopatía ; Cristalino patología ;

Localisation / Location

INIST-CNRS, Cote INIST : 21076, 35400007018774.0250