Titre du document / Document title
Construction of a novel beer proteome map and its use in beer quality control
Auteur(s) / Author(s)
IIMURE Takashi (1) ;
NANKAKU Nami (2) ;
HIROTA Naohiko (1) ;
TIANSU Zhou (1) ;
HOKI Takehiro (1) ;
KIHARA Makoto (1) ;
HAYASHI Katsuhiro (1) ;
ITO Kazutoshi (3) ;
SATO Kazuhiro (2) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Bioresources Research and Development Department, Sapporo Breweries Ltd., 37-1, Nittakizaki, Ota, Gunma 370-0393, JAPON
(2) Barley Germplasm Center. Research Institute for Bioresources, Okayama University, 2-20-1. Chuo, Kurashiki, Okayama 710-0046, JAPON
(3) Frontier Laboratories of Value Creation, Sapporo Breweries Ltd., 10 Okatohme, Yaizu, Shizuoka 425-0013, JAPON
Résumé / Abstract
Beer proteins were analysed by two-dimensional gel electrophoresis (2DE). The protein species associated with major spots on 2DE gels were identified by mass spectrometry followed by a database search to construct a comprehensive beer proteome map. As a result, 85 out of 199 protein spots examined were positively identified and categorised into 12 protein species. A total of 11 beer samples were brewed from the malt of eight cultivars having different levels of protein modification. This experiment was designed to demonstrate the influences of barley cultivar and malt modification on beer protein composition and beer quality characters. The beers produced from these brewing trails were subsequently analysed by 2DE and their proteomes were compared. Cultivars and malt modification affected the concentration of several proteins in beer. Beer protein concentration was associated with differences in the desirable beer quality trait, foam stability. In addition, expression of yeast derived proteins were observed that may also influence beer quality. Overall, the application of a comprehensive beer proteome map provides a strong platform for detection and potential manipulation of beer quality related proteins.
Revue / Journal Title
Food chemistry
ISSN
0308-8146
CODEN FOCHDJ
Source / Source
2010, vol. 118, n
o3, pp. 566-574 [9 page(s) (article)] (3/4 p.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, Kidlington, ROYAUME-UNI
(1976)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 17810, 35400019181271.0140
Nº notice refdoc (ud4) : 23100073
