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Titre du document / Document title

Development of a real-time PCR protocol for the species origin confirmation of isolated animal particles detected by NIRM

Auteur(s) / Author(s)

FUMIERE O. (1) ; MARIEN A. (1) ; FERNANDEZ PIERNA J. A. (1) ; BAETEN V. (1) ; BERBEN G. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Departement Valorisation des Productions agricoles, Centre wallon de Recherches agronomiques (CRA-W), Bâtiment Henseval, Chaussée de Namur, 24, 5030 Gembloux, BELGIQUE

Résumé / Abstract

At present, European legislation prohibits totally the use of processed animal proteins in feed for all farmed animals (Commission Regulation (EC) No. 1234/2003 ― extended feed ban). A softening of the feed ban for non-ruminants would nevertheless be considered if alternative methods could be used to gain more information concerning the species origin of processed animal proteins than that which can be provided by classical optical microscopy. This would allow control provisions such as the ban of feeding animals with proteins from the same species or intra-species recycling (Regulation (EC) No. 1774/2002). Two promising alternative methods, near-infrared microscopy (NIRM) and real-time polymerase chain reaction (PCR), were combined to authenticate, at the species level, the presence of animal particles. The paper describes the improvements of the real-time PCR method made to the DNA extraction protocol, allowing five PCR analyses to be performed with the DNA extracted from a single particle.

Revue / Journal Title

Food additives & contaminants. Part A. Chemistry, analysis, control, exposure & risk assessment    ISSN  1944-0049 

Source / Source

2010, vol. 27, no8, pp. 1118-1127 [10 page(s) (article)] (3/4 p.)

Langue / Language

Anglais

Editeur / Publisher

Taylor & Francis, Abingdon, ROYAUME-UNI  (2008) (Revue)

Mots-clés anglais / English Keywords

Feed

;

Molecular biology

;

Adulteration

;

Particle

;

Animal

;

Origin

;

Species

;

Polymerase chain reaction

;

Real time

;

Development

;

Mots-clés français / French Keywords

Aliment pour animal

;

Biologie moléculaire

;

Adultération

;

Particule

;

Animal

;

Origine

;

Espèce

;

Réaction chaîne polymérase

;

Temps réel

;

Développement

;

Mots-clés espagnols / Spanish Keywords

Alimento para animal

;

Biología molecular

;

Adulteración

;

Partícula

;

Animal

;

Origen

;

Especie

;

Reacción cadena polimerasa

;

Tiempo real

;

Desarrollo

;

Mots-clés d'auteur / Author Keywords

authenticity

;

molecular biology

;

polymerase chain reaction (PCR)

;

animal products

;

animal feedingstuffs

;

animal feed

;

Localisation / Location

INIST-CNRS, Cote INIST : 20834 A, 35400019168724.0080

Nº notice refdoc (ud4) : 23005994



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