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Titre du document / Document title

Production of solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst

Auteur(s) / Author(s)

KITTIPONGPATANA N. (1) ; HOCK R. S. (1) ; PORTER J. R. (1 2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Graduate Program in Medicinal Chemistry and Pharmacognosy; Department of Chemistry and Biochemistr, Philadelphia, PA 19104, ETATS-UNIS
(2) Graduate Program in Medicinal Chemistry and Pharmacognosy;Department of Biological Sciences, Philadelphia College of Pharmacy and Science, Philadelphia, PA 19104, ETATS-UNIS

Résumé / Abstract

The production of the steroidal alkaloid solasodine, an alternative to diosgenin as a precursor for the commercial production of steroid drugs, was studied in hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. through manipulation of culture medium. The individual and combined effects of medium components on the growth index and the production of solasodine were analyzed using factorial analysis of variance. Solasodine content was optimized to 6.2 mg g-1 in the hairy root, 1.4 mg g-' callus, and 0.7 mg g-1 in cell suspension cultures (dry weight). An improved isocratic reversed phase high performance liquid chromatographic method provided selective determination of the solasodine content of these samples. Analysis of growth and solasodine content of hairy root cultures and callus cultures demonstrated that the production of solasodine was shown to be growth-dependent in hairy root cultures but not in callus cultures.

Revue / Journal Title

Plant cell, tissue and organ culture    ISSN  0167-6857   CODEN PTCEDJ 

Source / Source

1998, vol. 52, no3, pp. 133-143 (24 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1981) (Revue)

Mots-clés anglais / English Keywords

Steroid

;

Alkaloid

;

Culture medium

;

Suspension culture

;

Tissue culture

;

Hairy root

;

Medium effect

;

Callus

;

Optimization

;

Vegetals

;

Medicinal plant

;

Production

;

Solanaceae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mots-clés français / French Keywords

Stéroïde

;

Alcaloïde

;

Milieu culture

;

Culture en suspension

;

Culture tissu

;

Hairy root

;

Effet milieu

;

Cal

;

Optimisation

;

Végétal

;

Plante médicinale

;

Production

;

Solanum aviculare

;

Solasodine

;

Solanaceae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mots-clés espagnols / Spanish Keywords

Esteroide

;

Alcaloide

;

Medio cultivo

;

Cultivo en suspensión

;

Cultivo tejido

;

Hairy root

;

Efecto medio

;

Callo

;

Optimización

;

Vegetal

;

Planta medicinal

;

Producción

;

Solanaceae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Localisation / Location

INIST-CNRS, Cote INIST : 19374, 35400007686042.0030

Nº notice refdoc (ud4) : 2299687



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