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Titre du document / Document title

International Standardisation of a Method for Detection of Human Pathogenic Viruses in Molluscan Shellfish

Auteur(s) / Author(s)

Lees David (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) European Community Reference Laboratory, Centre for Environment, Fisheries and Aquaculture Science, Dorset, The Nothe, Weymouth, UK

Résumé / Abstract

The viruses primarily associated with shellfish-borne illness are norovirus, causing gastroenteritis and hepatitis A virus (HAV). Recent years have seen a proliferation of publications on methods for detection of these viruses in shellfish using polymerase chain reaction (PCR). However, currently no standard harmonised procedures have been published. Standardisation is necessary before virus methods can be considered for adoption within a regulatory framework. A European standardisation working group is developing a two-part (quantitative and qualitative) standard method for virus detection in foodstuffs, including shellfish, which has the potential to be incorporated into EU legislation as a reference method. This article describes the development of the standard method and outlines the key methodology principles adopted, the controls and other quality assurance measures supporting the method and future necessary developments in the area.

Revue / Journal Title

Food and environmental virology    ISSN  1867-0342 

Source / Source

2010, vol. 2, no3, pp. 146-155 [10 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (2009) (Revue)

Mots-clés d'auteur / Author Keywords

Shellfish

;

Norovirus

;

Hepatitis A virus

;

Method

;

Detection

;

Standard

;

Localisation / Location

35400060558740.0005

Springer Science + Business Media, LLC, 2010
Nº notice refdoc (ud4) : 22938698



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