Titre du document / Document title
STRATEGIES FOR THE PRODUCTION OF MAIZE-DERIVED PHARMACEUTICALS USING CYTOPLASMIC MALE STERILE LINES: IN VITRO TISSUE CULTURE/TRANSFORMATION AND FIELD BREEDING APPROACHES
Auteur(s) / Author(s)
WANG K. (1 2) ;
FRAME B. (1 2) ;
XU X. (1 3) ;
MOELLER L. (1 3) ;
LAMKEY K. (1) ;
WISE R. (4) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Agronomy, Iowa State University, Ames, Iowa 50011, ETATS-UNIS
(2) Center for Plant Transformation, Plant Science Institute, Iowa State University, Ames, Iowa 50011, ETATS-UNIS
(3) Interdepartmental Plant Biology Major, Iowa State University, Ames, Iowa 50011, ETATS-UNIS
(4) Corn Insects and Crop Genetics Research, USDA-ARS, and Department of Plant Pathology, Iowa State University, Ames, IA, 50011-1020, ETATS-UNIS
Résumé / Abstract
Plant-made pharmaceuticals (PMPs) offer promise as efficient and cost-effective products for the treatment of human and animal diseases. An advantage of producing pharmaceuticals in maize is the large storage capacity and stability for proteins and starch in seed, allowing for manufacturing recomhinant proteins such as antigens and antibodies. Other advantages of the maize system include safety, high yields, and scalability of production and processing. However, the henefits of this technology must be balanced against potential health and environmental risks that may be associated with its use. Because PMPs presently have no provision for regulatory tolerance, their inadvertent occurrence in foods and feeds remains an important economic consideration, even when the health and environmental risks are low. Pollen drift is considered a source of potential contamination of maize-made pharmaceuticals in the food chain. In addition to physical and temporal isolation requirements, open field pharmaceutical maize production also calls for controlled pollen release. Here, we describe two strategies to address the issue of transgenic pollen drift. First, we describe the development and genetic transformation of a tissue culture-amenable male-sterile line using biolistic- or Agrobacterium-mediated transformation methods. Secondly, we describe the introgression of a transgene from male-fertile transgenic maize to male-sterile germplasm by conventional breeding. After six seasons of breeding, this second strategy allows us to obtain 100% transgenic seeds from an open-field production using a non-transgenic line as the pollinator.
Revue / Journal Title
Maydica
ISSN
0025-6153
CODEN MYDCAH
Source / Source
Congrès
EUCARPIA Maize and Sorghum International Conference N
o21, Bergamo
, ITALIE
(21/06/2009)
2009, vol. 54, n
o 2-3 (233 p.) [Document : 12 p.] (1 p.1/2), pp. 199-210 [12 page(s) (article)]
Langue / Language
Anglais
Editeur / Publisher
Maydica, Bergamo, ITALIE
(1956)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 12198, 35400018082215.0050
Nº notice refdoc (ud4) : 22421392
