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Titre du document / Document title

STRATEGIES FOR THE PRODUCTION OF MAIZE-DERIVED PHARMACEUTICALS USING CYTOPLASMIC MALE STERILE LINES: IN VITRO TISSUE CULTURE/TRANSFORMATION AND FIELD BREEDING APPROACHES

Auteur(s) / Author(s)

WANG K. (1 2) ; FRAME B. (1 2) ; XU X. (1 3) ; MOELLER L. (1 3) ; LAMKEY K. (1) ; WISE R. (4) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Agronomy, Iowa State University, Ames, Iowa 50011, ETATS-UNIS
(2) Center for Plant Transformation, Plant Science Institute, Iowa State University, Ames, Iowa 50011, ETATS-UNIS
(3) Interdepartmental Plant Biology Major, Iowa State University, Ames, Iowa 50011, ETATS-UNIS
(4) Corn Insects and Crop Genetics Research, USDA-ARS, and Department of Plant Pathology, Iowa State University, Ames, IA, 50011-1020, ETATS-UNIS

Résumé / Abstract

Plant-made pharmaceuticals (PMPs) offer promise as efficient and cost-effective products for the treatment of human and animal diseases. An advantage of producing pharmaceuticals in maize is the large storage capacity and stability for proteins and starch in seed, allowing for manufacturing recomhinant proteins such as antigens and antibodies. Other advantages of the maize system include safety, high yields, and scalability of production and processing. However, the henefits of this technology must be balanced against potential health and environmental risks that may be associated with its use. Because PMPs presently have no provision for regulatory tolerance, their inadvertent occurrence in foods and feeds remains an important economic consideration, even when the health and environmental risks are low. Pollen drift is considered a source of potential contamination of maize-made pharmaceuticals in the food chain. In addition to physical and temporal isolation requirements, open field pharmaceutical maize production also calls for controlled pollen release. Here, we describe two strategies to address the issue of transgenic pollen drift. First, we describe the development and genetic transformation of a tissue culture-amenable male-sterile line using biolistic- or Agrobacterium-mediated transformation methods. Secondly, we describe the introgression of a transgene from male-fertile transgenic maize to male-sterile germplasm by conventional breeding. After six seasons of breeding, this second strategy allows us to obtain 100% transgenic seeds from an open-field production using a non-transgenic line as the pollinator.

Revue / Journal Title

Maydica    ISSN  0025-6153   CODEN MYDCAH 

Source / Source

Congrès
EUCARPIA Maize and Sorghum International Conference No21, Bergamo , ITALIE (21/06/2009)
2009, vol. 54, no 2-3 (233 p.)  [Document : 12 p.] (1 p.1/2), pp. 199-210 [12 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Maydica, Bergamo, ITALIE  (1956) (Revue)

Mots-clés anglais / English Keywords

Cereal crop

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Biopharmaceuticals

;

Transgenic plant

;

Zea mays

;

Molecular farming

;

Male sterility

;

Genetic transformation

;

Tissue culture

;

In vitro

;

Cytoplasmic male sterility

;

Pharmacological activity

;

Mots-clés français / French Keywords

Plante céréalière

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Produit biopharmaceutique

;

Plante transgénique

;

Zea mays

;

Moléculture

;

Stérilité mâle

;

Transformation génétique

;

Culture tissu

;

In vitro

;

Stérilité mâle cytoplasmique

;

Propriété pharmacologique

;

Mots-clés espagnols / Spanish Keywords

Planta cerealista

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Planta transgénica

;

Zea mays

;

Agricultura molecular

;

Esterilidad masculina

;

Transformación genética

;

Cultivo tejido

;

In vitro

;

Esterilidad masculina citoplásmica

;

Propiedad farmacológica

;

Mots-clés d'auteur / Author Keywords

Genetic transformation

;

Male-sterile

;

Plant-made pharmaceuticals

;

Zea mays

;

Localisation / Location

INIST-CNRS, Cote INIST : 12198, 35400018082215.0050

Nº notice refdoc (ud4) : 22421392



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