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Titre du document / Document title

Defining the Window of Germline Genesis In Vitro from Murine Embryonic Stem Cells

Auteur(s) / Author(s)

YOUNG Julia C. (1 2) ; DIAS Vinali L. (2) ; LOVELAND Kate L. (1 2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) ARC Centre of Excellence in Biotechnology and Development, Monash University, Clayton, Victoria, AUSTRALIE
(2) School of Biomedical Science, Monash University, Clayton, Victoria, AUSTRALIE

Résumé / Abstract

Bone morphogenetic protein (BMP) signaling is critical for germline establishment during mouse embryogenesis. To exploit its importance for induction of germline precursors in vitro, mouse embryonic stem cells (mESCs) were cultured as embryoid body (EB) aggregates with combinations of BMP2, BMP4, and BMP8B for 3-10 days. At Day 10 of culture, well-delineated clusters of POU5F1-positive (POU5F1+) cells were visible in BMP4-treated and BMP2-treated EBs; these were rarely detected in untreated and BMP8B-treated cultures. Quantitative mRNA analysis revealed that a significant elevation of markers associated with primordial germ cell development had occurred in the presence of BMP4 by Day 10, including late germline markers such as Ddx4 (Mvh). Reasoning that germline specification was established by Day 10, we surveyed earlier time points for altered levels of germline marker mRNAs. A peak of early markers, Prdm1 (Blimp1), lfitm3 (Fragilis), and Dppa3 (Stella), was measured in Day 3 to Day 4 EBs grown in BMP4, followed by a decrease at Day 5. In contrast, other markers, Pou5f1, Nanog, Dazl, and Ddx4, progressively increased from Day 3 to Day 5. Transforming growth factor beta superfamily signaling components Acvr1 (ALK2), Smad1, and Smad5 remained relatively constant. Isolated POU5F1+ cells from BMP4-treated Day 5 EBs contained significantly elevated germline markers compared with POU5F1-negative cells, with a transcript profile differing from mESCs, verifying their unique identity. These results demonstrate that signaling by BMP2 and BMP4, but not BMP8B, enhances germline marker expression within EBs and identify Day 3 to Day 5 in EB differentiation as a window for specification of germ cells in vitro.

Revue / Journal Title

Biology of reproduction    ISSN  0006-3363   CODEN BIREBV 

Source / Source

2010, vol. 82, no2, pp. 390-401 [12 page(s) (article)] (46 ref.)

Langue / Language

Anglais

Editeur / Publisher

Society for the Study of Reproduction, Madison, WI, ETATS-UNIS  (1969) (Revue)

Mots-clés anglais / English Keywords

Reproduction

;

Germinal cell

;

Early stage

;

Differentiation

;

Stem cell

;

Embryo

;

In vitro

;

Mots-clés français / French Keywords

Reproduction

;

Cellule germinale

;

Stade précoce

;

Différenciation

;

Cellule souche

;

Embryon

;

In vitro

;

Mots-clés espagnols / Spanish Keywords

Reproducción

;

Célula germinal

;

Estadio precoz

;

Diferenciación

;

Célula primitiva

;

Embrión

;

In vitro

;

Mots-clés d'auteur / Author Keywords

differentiation

;

early development

;

embryoid body

;

embryonic stem cell

;

primordial germ cell

;

TGFB superfamily

;

Localisation / Location

INIST-CNRS, Cote INIST : 14393, 35400018093618.0190

Nº notice refdoc (ud4) : 22380101



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