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Titre du document / Document title

Syringetin, a flavonoid derivative in grape and wine, induces human osteoblast differentiation through bone morphogenetic protein-2/extracellular signal-regulated kinase 1/2 pathway

Auteur(s) / Author(s)

HSU Ya-Ling (1) ; LIANG Hsin-Lin (2 3) ; HUNG Chih-Hsing (1 4) ; KUO Po-Lin (5 6) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, TAIWAN, PROVINCE DE CHINE
(2) Department of Pharmacology, Kaohsiung Municipal United Hospital, Kaohsiung, TAIWAN, PROVINCE DE CHINE
(3) School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, TAIWAN, PROVINCE DE CHINE
(4) Department of Pediatrics, Faculty of Pediatrics, Kaohsiung Medical University Hospital, Kaohsiung, TAIWAN, PROVINCE DE CHINE
(5) Institute of Clinical Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, TAIWAN, PROVINCE DE CHINE
(6) Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, TAIWAN, PROVINCE DE CHINE

Résumé / Abstract

Syringetin (3,5,7,4'-tetrahydroxy-3',5'dimethoxyflavone), a flavonoid derivative, is present in grape and wine. By means of alkaline phosphatase (ALP) activity, osteocalcin, and type I collagen ELISA, we have shown that syringetin exhibits a significant induction of differentiation in MC3T3-E1 mouse calvaria osteoblasts and human fetal osteoblastic 1.19 cell line human osteoblasts. ALP and osteocalcin are phenotypic markers for early-stage differentiated osteoblasts and terminally differentiated osteoblasts, respectively. Our results indicate that syringetin stimulates osteoblast differentiation at various stages, from maturation to terminally differentiated osteoblasts. Induction of differentiation by syringetin is associated with increased bone morphogenetic protein-2 (BMP-2) production. The BMP-2 antagonist noggin blocked syringetin-mediated ALP activity and osteocalcin secretion enhancement, indicating that BMP-2 production is required in syringetin-mediated osteoblast maturation and differentiation. Induction of differentiation by syringetin is associated with increased activation of SMAD1/5/8 and extracellular signal-regulated kinase 1/2 (ERK1/2). Cotreatment of ERK1/2 inhibitor 2'-amino-3'-methoxyflavone inhibited syringetin-mediated ALP upregulation and osteocalcin production. In conclusion, syringetin increased BMP-2 synthesis, and subsequently activated SMAD1/5/8 and ERK1/2, and this effect may contribute to its action on the induction of osteoblast maturation and differentiation, followed by an increase of bone mass.

Revue / Journal Title

Molecular nutrition & food research    ISSN  1613-4125 

Source / Source

2009, vol. 53, no11, pp. 1452-1461 [10 page(s) (article)] (41 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wiley, Weinheim, ALLEMAGNE  (2004) (Revue)

Mots-clés anglais / English Keywords

Alcoholic beverage

;

Fruit

;

Polyphenol

;

Extracellular

;

Protein

;

Bone

;

Differentiation

;

Human

;

Wine

;

Grape

;

Flavonoid

;

Mots-clés français / French Keywords

Boisson alcoolisée

;

Fruit

;

Polyphénol

;

Extracellulaire

;

Protéine

;

Os

;

Différenciation

;

Homme

;

Vin

;

Raisin

;

Flavonoïde

;

Mots-clés espagnols / Spanish Keywords

Bebida alcohólica

;

Fruto

;

Polifenol

;

Extracelular

;

Proteína

;

Hueso

;

Diferenciación

;

Hombre

;

Vino

;

Uva

;

Flavonoide

;

Mots-clés d'auteur / Author Keywords

Bone morphogenetic protein-2

;

Differentiation

;

Extracellular signal-regulated kinase1/2

;

Osteoblasts

;

Syringetin

;

Localisation / Location

INIST-CNRS, Cote INIST : 9262, 35400017144859.0100

Nº notice refdoc (ud4) : 22159156



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