Titre du document / Document title

The application of high performance liquid chromatography, coupled to nuclear magnetic resonance spectroscopy and mass spectrometry (HPLC-NMR-MS), to the characterisation of ibuprofen metabolites from human urine

Auteur(s) / Author(s)

CLAYTON E. ⁽¹⁾ ; TAYLOR S. ⁽¹⁾ ; WRIGHT B. ⁽¹⁾ ; WILSON I. D. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Zeneca Pharmaceuticals, Mereside, Alderley Park, Macclesfield, Cheshire SK10 4TG, ROYAUME-UNI

Résumé / Abstract

The use of HPLC-NMR-MS for the detection and identification of the metabolites of ibuprofen present in a solid phase extract of human urine is described. Gradient reversed-phase HPLC was used to separate the components present in the extract, which were then characterised by a combination of stopped-flow ¹H NMR and on line electrospray-MS. This approach led to the rapid identification of the known phase 1 human metabolites of ibuprofen, including hydroxy- and carboxy- metabolites, together with their respective glucuronide conjugates. In addition a probable artefact resulting from the dehydration of one of the side chain-hydroxylated glucuronides was also identified.

Revue / Journal Title

Chromatographia   ISSN 0009-5893   CODEN CHRGB7 

Source / Source

1998, vol. 47, n^o5-6, pp. 264-270 (11 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Oxford, ROYAUME-UNI  (1968) (Revue)

Mots-clés anglais / English Keywords

Chemical analysis ; Qualitative analysis ; Metabolite ; Ibuprofen ; Arylpropionic acid derivatives ; Biological fluid ; Urine ; Human ; Sample preparation ; Solid phase extraction ; HPLC chromatography ; Reversed phase chromatography ; Gradient ; Elution ; Stopped flow method ; Coupled method ; Mass spectrometry ; Electrospray ; NMR spectrometry ; Hydrogen 1 ;

Mots-clés français / French Keywords

Analyse chimique ; Analyse qualitative ; Métabolite ; Ibuprofène ; Arylpropionique acide dérivé ; Liquide biologique ; Urine ; Homme ; Préparation échantillon ; Extraction SPE ; Chromatographie HPLC ; Chromatographie phase inverse ; Gradient ; Elution ; Méthode stopped flow ; Méthode couplée ; Spectrométrie masse ; Electrospray ; Spectrométrie RMN ; Hydrogène 1 ;

Mots-clés espagnols / Spanish Keywords

Análisis químico ; Análisis cualitativo ; Metabolito ; Ibuprofeno ; Arilpropionico ácido derivado ; Líquido biológico ; Orina ; Hombre ; Preparación muestreo ; Extracción SPE ; Cromatografía HPLC ; Cromatografía fase inversa ; Gradiente ; Elución ; Método flúido detenido ; Método acoplado ; Espectrometría masa ; Electrospray ; Espectrometría RMN ;

Localisation / Location

INIST-CNRS, Cote INIST : 8489, 35400007900088.0030