Titre du document / Document title

Isolation and sources of 'blown pack' spoilage clostridia in beef abattoirs

Auteur(s) / Author(s)

MOSCHONAS G. ^{(1 2)} ; BOLTON D. J. ⁽¹⁾ ; SHERIDAN J. J. ⁽¹⁾ ; MCDOWELL D. A. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Ashtown Food Research Centre, Teagasc, Dublin, IRLANDE
⁽²⁾ School of Health Sciences, University of Ulster, Newtownabbey, Co, Antrim, ROYAUME-UNI

Résumé / Abstract

Aims: (i) To evaluate methods for isolation and molecular detection of blown pack spoilage (BPS) clostridia and (ii) to survey beef abattoirs for sources and distributions of Clostridium estertheticum and Cl. gasigenes. Methods and Results: Molecular detection and conventional isolation methods were used to detect and recover BPS associated clostridia (Cl. estertheticum and Cl. gasigenes), from four commercial Irish beef abattoirs and their environments, during a one year study. DNA-based methods detected 218 Cl. estertheticum and 300 Cl. gasigenes, from 1680 samples, whereas culture-methods only yielded 17 Cl. estertheticum and 176 Cl. gasigenes isolates. BPS Clostridia were frequently detected in beef abattoirs and their environments, especially at areas prior to hide removal. The study noted a higher percentage of positive samples during the month of May (38·6%). Conclusions: (i) DNA-based techniques are the most reliable ways to determine the presence of these organisms in various samples and (ii) hides and faeces are the main reservoirs of BPS clostridia in the abattoirs. Significance and Impact of the Study: This paper provides useful information to detect BPS organisms, as well as to develop a science-based control strategy of the problem.

Revue / Journal Title

Journal of applied microbiology   ISSN 1364-5072 

Source / Source

2009, vol. 107, n^o2, pp. 616-624 [9 page(s) (article)] (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Blackwell Science, Oxford, ROYAUME-UNI  (1997) (Revue)

Mots-clés anglais / English Keywords

Bacteria ; Clostridiales ; Clostridiaceae ; Applied microbiology ; Psychrophily ; Clostridium ; Beef ; Biological contamination ; Source ; Isolation ;

Mots-clés français / French Keywords

Bactérie ; Clostridiales ; Clostridiaceae ; Microbiologie appliquée ; Psychrophilie ; Clostridium ; Viande boeuf ; Contamination biologique ; Source ; Isolement ;

Mots-clés espagnols / Spanish Keywords

Bacteria ; Clostridiales ; Clostridiaceae ; Microbiología aplicada ; Psicrofilia ; Clostridium ; Carne vaca ; Contaminación biológica ; Fuente ; Aislamiento ;

Mots-clés d'auteur / Author Keywords

abattoir ; blown pack spoilage ; Clostridium ; psychrophilic ;

Localisation / Location

INIST-CNRS, Cote INIST : 7415, 35400017242141.0280